Oberhofer T R, Rowen J W, Cunningham G F, Higbee J W
J Clin Microbiol. 1977 Dec;6(6):559-66. doi: 10.1128/jcm.6.6.559-566.1977.
The Oxi/Ferm test system was evaluated for accuracy and reliability for identification of nonfermentative and oxidase-positive fermentative bacteria by using 375 bacterial strains obtained from stock culture and clinical specimens. The Oxi/Ferm system is a compartmentalized tube containing eight media to provide nine biochemical test results. When combined with the oxidase test, the results corresponding to the positive reactions are totaled and the composite number is located in the coding manual to identify the organisms. The 375 isolates studied were evaluated for accuracy of identification, using both the original and revised code manuals. In comparison with the conventional media used, there was 100% correlation in tests for hydrogen sulfide and indole production, over 96% for nitrogen gas, arginine, and urease, over 92% for xylose and dextrose oxidation, and less than 90% for citrate utilization and dextrose fermentation. There was an overall accuracy in identification of 89.3% using the original manual, with accuracy revised slightly upward to 90.7% using the revised manual. There was 100% accuracy in identification with 44.0% of the strains tested (11 species) using the original manual and with 66.1% (16 species) using the revised manual. Thirteen of the 40 original misidentifications and 14 of 35 revised misidentifications resulted from failure to code and were unidentifiable by Oxi/Ferm. The remainder were incorrectly identified or could not be differentiated from closely related strains. Eleven strains of Alcaligenes odorans were correctly identified using the original code, whereas no code was provided in the revised manual. The Oxi/Ferm system is both simple and rapid and is satisfactory for identification of the more common isolates.
通过使用从保藏培养物和临床标本中获得的375株细菌菌株，对Oxi/Ferm检测系统在鉴定非发酵菌和氧化酶阳性发酵菌方面的准确性和可靠性进行了评估。Oxi/Ferm系统是一种分隔管，包含八种培养基，可提供九种生化检测结果。当与氧化酶检测相结合时，将对应阳性反应的结果相加，并在编码手册中查找复合数字以鉴定微生物。使用原始和修订后的编码手册，对所研究的375株分离株进行了鉴定准确性评估。与使用的传统培养基相比，硫化氢和吲哚产生试验的相关性为100%，氮气、精氨酸和尿素酶试验的相关性超过96%，木糖和葡萄糖氧化试验的相关性超过92%，柠檬酸盐利用和葡萄糖发酵试验的相关性低于90%。使用原始手册时，总体鉴定准确率为89.3%，使用修订手册时准确率略有提高至90.7%。使用原始手册时，44.0%（11种）的测试菌株鉴定准确率为100%，使用修订手册时为66.1%（16种）。40例原始错误鉴定中的13例和35例修订后错误鉴定中的14例是由于未能编码而无法通过Oxi/Ferm鉴定。其余的要么鉴定错误，要么无法与密切相关的菌株区分开来。使用原始编码正确鉴定了11株恶臭产碱菌，而修订后的手册中未提供编码。Oxi/Ferm系统既简单又快速，对于鉴定较常见的分离株令人满意。
