Zheng J, Tan T, Kuang A
Department of Nuclear Medicine, First Affiliated Hospital of West China University of Medical Sciences, Chengdu 610041.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 1999 Dec;16(4):516-9, 523.
Antisense technique, developed on the principle of base complementation, is a method using short strand oligonucleotide fragments complementary to target DNAs or target RANs for inhibition of gene expression. As a theoretically perfect and specific method to block the expression of gene, it has been rapidly developed over the last twenty years. This review expatiates on its techniques, its problem and possible way of problem-solving, and its recent application in medicine. Also this review discusses the trends of its further development.
反义技术是基于碱基互补原理发展起来的，它是一种利用与靶DNA或靶RNA互补的短链寡核苷酸片段来抑制基因表达的方法。作为一种从理论上看完美且特异的阻断基因表达的方法，在过去二十年里它得到了迅速发展。本文综述阐述了其技术、存在的问题及可能的解决途径，以及其在医学领域的最新应用。此外，本文还讨论了其进一步发展的趋势。
