早期生长反应因子-1作为胃癌转移调节因子的过表达
Overexpression of early growth response-1 as a metastasis-regulatory factor in gastric cancer.
作者信息
Kobayashi Daisuke, Yamada Mikako, Kamagata Chinatsu, Kaneko Reiko, Tsuji Naoki, Nakamura Masashi, Yagihashi Atsuhito, Watanabe Naoki
机构信息
Department of Clinical Laboratory Medicine, Division of Laboratory Diagnosis, Sapporo Medical University School of Medicine, South-1, West-16, Chuo-ku, Sapporo 060-8543, Japan.
出版信息
Anticancer Res. 2002 Nov-Dec;22(6C):3963-70.
BACKGROUND
To investigate the potential role of a nuclear transcription factor, early growth response-1 (Egr-1), in formation and progression of gastric cancer, we compared its expression in gastric cancers with that in non-cancerous tissues.
MATERIALS AND METHODS
Egr-1 mRNA expression was measured using TaqMan RT-PCR. The corresponding protein expression was examined immunohistochemically.
RESULTS
Egr-1 mRNA expression was significantly higher in gastric cancer tissues than in normal mucosa (p < 0.0005). These differences were also reflected by protein product expression. Moreover, Egr-1 mRNA expression was higher in cases with metastasis to lymph nodes or remote organs. In cultured gastric cancer cells known to have a high metastatic potential, expression of this mRNA was higher than that of parental cells.
CONCLUSION
It was suggested that Egr-1 has a significant role in carcinogenesis and in cancer progression, especially metastasis. Measurement of this mRNA should be useful for evaluation of the metastatic potential of gastric cancer.
背景
为了研究核转录因子早期生长反应因子1（Egr-1）在胃癌形成和进展中的潜在作用，我们比较了其在胃癌组织与非癌组织中的表达情况。
材料与方法
使用TaqMan RT-PCR检测Egr-1 mRNA的表达。采用免疫组织化学方法检测相应的蛋白表达。
结果
Egr-1 mRNA在胃癌组织中的表达显著高于正常黏膜（p < 0.0005）。这些差异也反映在蛋白产物表达上。此外，Egr-1 mRNA在发生淋巴结转移或远处器官转移的病例中表达更高。在已知具有高转移潜能的培养胃癌细胞中，该mRNA的表达高于亲本细胞。
结论
提示Egr-1在致癌作用以及癌症进展尤其是转移过程中具有重要作用。检测这种mRNA对于评估胃癌的转移潜能应该是有用的。
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