Akiba Masato, Uchida Ikuo, Nishimori Kei, Tanaka Kiyoshi, Anzai Toru, Kuwamoto Yasushi, Wada Ryuichi, Ohya Tatsuo, Ito Hiroya
Kyusyu Research Station, National Institute of Animal Health, 2702 Chuzan-cho, Kagoshima-shi, Japan.
Vet Microbiol. 2003 Apr 29;92(4):379-88. doi: 10.1016/s0378-1135(02)00422-4.
Equine paratyphoid is caused by Salmonella enterica serovar Abortusequi, and manifests mainly as abortion in the mare. We compared S. Abortusequi strains isolated in Japan and other countries using pulsed-field gel electrophoresis (PFGE) and fluorescent amplified-fragment length polymorphism (FAFLP) analysis. PFGE analysis of S. Abortusequi strains gave 21-27 fragments ranging in size from 33 to 602kb. Although two PFGE profiles were observed among the 20 S. Abortusequi isolates in Japan, the restriction fragments originating from the chromosome were common between the two profiles. The similarity index of the two profiles was 90.9%, while those between Japanese and five other S. Abortusequi strains were 29.8-37.5%. On the other hand, FAFLP analysis of S. Abortusequi strains generated 64-67 amplified fragments ranging in size from 100 to 400bp. One polymorphic fragment was observed among the 20 S. Abortusequi isolates in Japan. These data indicate the close relation of this agent in Japan. S. Abortusequi strains sharing a common ancestry might have been conserved in Japan.
马副伤寒由肠炎沙门氏菌马流产亚种引起，主要表现为母马流产。我们使用脉冲场凝胶电泳（PFGE）和荧光扩增片段长度多态性（FAFLP）分析，比较了在日本和其他国家分离出的马流产沙门氏菌菌株。对马流产沙门氏菌菌株进行PFGE分析得到21 - 27个片段，大小在33至602kb之间。尽管在日本的20株马流产沙门氏菌分离株中观察到两种PFGE图谱，但两种图谱中源自染色体的限制性片段是相同的。两种图谱的相似性指数为90.9%，而日本的菌株与其他五株马流产沙门氏菌菌株之间的相似性指数为29.8 - 37.5%。另一方面，对马流产沙门氏菌菌株进行FAFLP分析产生了64 - 67个扩增片段，大小在100至400bp之间。在日本的20株马流产沙门氏菌分离株中观察到一个多态性片段。这些数据表明该病原体在日本存在密切关系。具有共同祖先的马流产沙门氏菌菌株可能在日本得到了保存。
