Niwa Hidekazu, Hobo Seiji, Kinoshita Yuta, Muranaka Masanori, Ochi Akihiro, Ueno Takanori, Oku Kazuomi, Hariu Kazuhisa, Katayama Yoshinari
The Enzootic Research Center, Equine Research Institute, Japan Racing Association, Tochigi, Japan (Niwa, Kinoshita, Muranaka, Ochi, Ueno, Oku, Hariu, Katayama)Department of Domestic Animal Internal Medicine, Clinical Veterinary Science, Veterinary Medicine, Joint Faculty of Veterinary Medicine, Kagoshima University, Japan (Hobo)
The Enzootic Research Center, Equine Research Institute, Japan Racing Association, Tochigi, Japan (Niwa, Kinoshita, Muranaka, Ochi, Ueno, Oku, Hariu, Katayama)Department of Domestic Animal Internal Medicine, Clinical Veterinary Science, Veterinary Medicine, Joint Faculty of Veterinary Medicine, Kagoshima University, Japan (Hobo).
J Vet Diagn Invest. 2016 Jul;28(4):440-4. doi: 10.1177/1040638716649640. Epub 2016 Jun 6.
Salmonella enterica subsp. enterica serovar Abortusequi is a pathogen restricted to horses. Our investigation targeted 4 draft horses (9-10 months old) kept on a Japanese farm that had suffered an outbreak of S. Abortusequi abortion. The 4 horses were suspected to be carriers of the bacterium owing to their high agglutination titers (≥1:2,560) in tube agglutination testing. The owners' on-farm observations confirmed that the horses had no apparent abnormalities, and S. Abortusequi was not isolated from their blood, rectal swabs, or sternal bone marrow fluid at antemortem investigation. However, at autopsy, all horses displayed the following: suppurative aneurysm of the cranial mesenteric artery with heavy infection with Strongylus vulgaris larvae; heavy intestinal parasitic infection with Gasterophilus intestinalis, Parascaris equorum, Anoplocephala perfoliata, and S. vulgaris; and enlargement of the systemic lymph nodes. In each case, large numbers of S. Abortusequi were isolated from the anterior mesenteric artery thrombus. The thrombus isolates harbored a single virulence plasmid, and the pulsed-field gel electrophoresis profiles of the isolates were identical not only to each other but also to those of Japanese enzootic strains of S. Abortusequi. These results reveal that parasitic aneurysms of the cranial mesenteric artery should be considered an important possible site of carriage of S. Abortusequi in horses. The results also suggest high clonality of the isolated serovar in the horse population in Japan.
肠炎沙门氏菌肠炎亚种马流产沙门氏菌是一种仅感染马的病原体。我们的调查对象是日本一个农场饲养的4匹役用马（9 - 10个月大），该农场曾爆发马流产沙门氏菌流产疫情。这4匹马在试管凝集试验中凝集效价较高（≥1:2,560），被怀疑是该细菌的携带者。主人在农场的观察证实这些马没有明显异常，并且在生前调查中，未从它们的血液、直肠拭子或胸骨骨髓液中分离出马流产沙门氏菌。然而，在尸检时，所有马匹均表现出以下情况：颅肠系膜动脉化脓性动脉瘤，伴有严重的普通圆线虫幼虫感染；严重的肠道寄生虫感染，包括肠胃蝇、马副蛔虫、叶状裸头绦虫和普通圆线虫；以及全身淋巴结肿大。在每例病例中，从前肠系膜动脉血栓中均分离出大量马流产沙门氏菌。血栓分离株携带一个单一的毒力质粒，这些分离株的脉冲场凝胶电泳图谱不仅彼此相同，而且与日本马流产沙门氏菌地方流行株的图谱相同。这些结果表明，颅肠系膜动脉的寄生性动脉瘤应被视为马流产沙门氏菌在马体内重要的可能携带部位。这些结果还表明，在日本马群中分离出的该血清型具有高度的克隆性。
