Valentine J L, Driscoll P, Hamburg E L, Thompson E D
J Pharm Sci. 1976 Jan;65(1):96-8. doi: 10.1002/jps.2600650120.
Human plasma was made alkaline and extracted with methylene chloride. To the extract was added the internal standard, cinchonine, followed by evaporation to dryness. The resultant residue was dissolved in a methanolic solution containing trimethylanilinium hydroxide. This solution was assayed by GLC for quinidines (quinidine and hydroquinidine). Evaluation of the method over a 0.5-10-mug/ml range in human plasma gave an overall precision and accuracy of +/- 4.5% (RSD and RE). Plasma of several patients was analyzed by the present method as well as by a fluorometric method for the level of quinidines. Results from the two methods were comparable.
将人血浆碱化，并用二氯甲烷萃取。向萃取液中加入内标辛可宁，然后蒸发至干。将所得残渣溶于含氢氧化三甲基苯胺的甲醇溶液中。该溶液用气相色谱法测定奎尼丁（奎尼丁和氢奎尼丁）。在人血浆0.5 - 10μg/ml范围内对该方法进行评估，总体精密度和准确度为±4.5%（相对标准偏差和相对误差）。用本法以及荧光法分析了数名患者血浆中的奎尼丁水平。两种方法的结果具有可比性。
