Tan M H, Holyoke E D, Goldrosen M H
J Natl Cancer Inst. 1976 Apr;56(4):871-3. doi: 10.1093/jnci/56.4.871.
Two murine colon adenocarcinoma cell lines were established from primary cultures. The MCA-38 cell line was begun by treatment of the primary culture with trypsin to remove the fibroblastoid elements. The MCA-36 epithelial cells were sensitive to trypsin; therefore, the growth medium of MCA-36 primary cultures was augmented with collagenase to release the tumor-cell elements from the fibroblast network. These tumor elements were dissociated with trypsin and placed in tissue culture. Each cell line was cultured for at least 10 passages in vitro and gave rise to tumors when reimplanted in vivo.
从原代培养物中建立了两种小鼠结肠腺癌细胞系。MCA - 38细胞系是通过用胰蛋白酶处理原代培养物以去除成纤维样成分而建立的。MCA - 36上皮细胞对胰蛋白酶敏感；因此，在MCA - 36原代培养物的生长培养基中添加胶原酶，以从成纤维细胞网络中释放肿瘤细胞成分。这些肿瘤成分用胰蛋白酶解离后置于组织培养中。每个细胞系在体外培养至少10代，重新植入体内时会形成肿瘤。
