Melek F R, Miyase Toshio, Ghaly N S
Chemistry of Natural Products Department, National Research Centre, Dokki, 12622, Cairo, Egypt.
Phytochemistry. 2003 Feb;62(4):557-62. doi: 10.1016/s0031-9422(02)00386-2.
Four new oleanane-type saponins and a known one were isolated from the leaves and stems of Meryta lanceolata. The new saponins were characterised by spectroscopic means and chemical hydrolysis as 3-O-[beta-D-glucopyranosyl-(1-->3)-beta-D-glucopyranosyl-(1-->3)-[beta-D-glucopyranosyl-(1-->2)]-alpha-L-arabinopyranosyl]oleanolic acid 28-O-[alpha-L-rhamnopyranosyl-(1-->4)-beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranosyl] ester, 3-O-[beta-D- glucopyranosyl-(1-->3)-beta-D-glucopyranosyl-(1-->3)-[beta-D-glucopyranosyl-(1-->2)]-alpha-L-arabinopyranosyl]oleanolic acid 28-O-[alpha-L-rhamnopyranosyl-(1-->4)-beta-D-6-O-acetyl glucopyranosyl-(1-->6)-beta-D-glucopyranosyl] ester, 3-O-[beta-D-glucopyranosyl-(1-->2)-beta-D-glucopyranosyl-(1-->3)-beta-D-glucopyranosyl-(1-->3)-alpha-L-arabinopyranosyl]oleanolic acid 28-O-[alpha-L-rhamnopyranosyl-(1-->4)-beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranosyl] ester and 3-O-[beta-D-glucopyranosyl-(1-->2)-beta-D-glucopyranosyl-(1-->3)-beta-D-glucopyranosyl-(1-->3)-alpha-L-arabinopyranosyl]echinocystic acid 28-O-[alpha-L-rhamnopyranosyl-(1-->4)-beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranosyl] ester. The NMR assignments were made by means of HOHAHA, 1H-1H COSY, HMQC, HMBC and NOE difference studies.
从披针叶刺轴榈的叶和茎中分离出4个新的齐墩果烷型皂苷和1个已知皂苷。通过光谱手段和化学水解对新皂苷进行表征，分别为3 - O - [β - D - 吡喃葡萄糖基 - (1→3) - β - D - 吡喃葡萄糖基 - (1→3) - [β - D - 吡喃葡萄糖基 - (1→2)] - α - L - 阿拉伯吡喃糖基]齐墩果酸28 - O - [α - L - 鼠李吡喃糖基 - (1→4) - β - D - 吡喃葡萄糖基 - (1→6) - β - D - 吡喃葡萄糖基]酯、3 - O - [β - D - 吡喃葡萄糖基 - (1→3) - β - D - 吡喃葡萄糖基 - (1→3) - [β - D - 吡喃葡萄糖基 - (1→2)] - α - L - 阿拉伯吡喃糖基]齐墩果酸28 - O - [α - L - 鼠李吡喃糖基 - (1→4) - β - D - 6 - O - 乙酰基吡喃葡萄糖基 - (1→6) - β - D - 吡喃葡萄糖基]酯、3 - O - [β - D - 吡喃葡萄糖基 - (1→2) - β - D - 吡喃葡萄糖基 - (1→3) - β - D - 吡喃葡萄糖基 - (1→3) - α - L - 阿拉伯吡喃糖基]齐墩果酸28 - O - [α - L - 鼠李吡喃糖基 - (1→4) - β - D - 吡喃葡萄糖基 - (1→6) - β - D - 吡喃葡萄糖基]酯以及3 - O - [β - D - 吡喃葡萄糖基 - (1→2) - β - D - 吡喃葡萄糖基 - (1→3) - β - D - 吡喃葡萄糖基 - (1→3) - α - L - 阿拉伯吡喃糖基]刺囊酸2� - O - [α - L - 鼠李吡喃糖基 - (1→4) - β - D - 吡喃葡萄糖基 - (1→6) - β - D - 吡喃葡萄糖基]酯。通过全同核Hartmann - Hahn光谱(HOHAHA)、1H - 1H化学位移相关谱(COSY)、异核多量子相干谱(HMQC)、异核多键相关谱(HMBC)和核Overhauser效应(NOE)差值研究进行核磁共振(NMR)归属。
