Hamada Taku, Sasaki Hideki, Hayashi Tatsuya, Moritani Toshio, Nakao Kazuwa
Laboratory of Applied Physiology, Kyoto University Graduate School of Human and Environmental Studies, Kyoto 606-8501, Japan.
J Appl Physiol (1985). 2003 Jun;94(6):2107-12. doi: 10.1152/japplphysiol.00486.2002. Epub 2003 Jan 31.
There is considerable evidence to suggest that electrical stimulation (ES) activates glucose uptake in rodent skeletal muscle. It is, however, unknown whether ES can lead to similar metabolic enhancement in humans. We employed low-frequency ES through surface electrodes placed over motor points of quadriceps femoris muscles. In male subjects lying in the supine position, the highest oxygen uptake was obtained by a stimulation pattern with 0.2-ms biphasic square pulses at 20 Hz and a 1-s on-off duty cycle. Oxygen uptake was increased by approximately twofold throughout the 20-min stimulation period and returned to baseline immediately after stimulation. Concurrent elevation of the respiratory exchange ratio and blood lactate concentration indicated anaerobic glycogen breakdown and utilization during ES. Whole body glucose uptake determined by the glucose disposal rate during euglycemic clamp was acutely increased by 2.5 mg. kg(-1). min(-1) in response to ES and, moreover, remained elevated by 3-4 mg. kg(-1). min(-1) for at least 90 min after cessation of stimulation. Thus the stimulatory effect of ES on whole body glucose uptake persisted not only during, but also after, stimulation. Low-frequency ES may become a useful therapeutic approach to activate energy and glucose metabolism in humans.
有大量证据表明，电刺激（ES）可激活啮齿动物骨骼肌中的葡萄糖摄取。然而，ES是否能在人类中导致类似的代谢增强尚不清楚。我们通过置于股四头肌运动点上方的表面电极采用低频ES。在仰卧位的男性受试者中，通过以20 Hz的0.2毫秒双相方波脉冲和1秒通断占空比的刺激模式获得了最高摄氧量。在整个20分钟的刺激期内，摄氧量增加了约两倍，并在刺激后立即恢复到基线水平。呼吸交换率和血乳酸浓度的同时升高表明在ES期间存在无氧糖原分解和利用。在正常血糖钳夹期间通过葡萄糖处置率测定的全身葡萄糖摄取量因ES而急性增加2.5 mg·kg⁻¹·min⁻¹，此外，在刺激停止后至少90分钟内仍升高3 - 4 mg·kg⁻¹·min⁻¹。因此，ES对全身葡萄糖摄取的刺激作用不仅在刺激期间持续，而且在刺激后也持续。低频ES可能成为激活人类能量和葡萄糖代谢的一种有用的治疗方法。
