Liu Lei, Yermolaieva Olena, Johnson Wayne A, Abboud Francois M, Welsh Michael J
Department of Internal Medicine, University of Iowa, Roy J. and Lucille A. Carver College of Medicine, 500 EMRB, Iowa City, Iowa 52242, USA.
Nat Neurosci. 2003 Mar;6(3):267-73. doi: 10.1038/nn1009.
Although the ability to sense temperature is critical for many organisms, the underlying mechanisms are poorly understood. Using the calcium reporter yellow cameleon 2.1 and electrophysiological recordings, we identified thermosensitive neurons and examined their physiologic response in Drosophila melanogaster larvae. In the head, terminal sensory organ neurons showed increased activity in response to cooling by < or =1 degrees C, heating reduced their basal activity, and different units showed distinct response patterns. Neither cooling nor heating affected dorsal organ neurons. Body wall neurons showed a variety of distinct response patterns to both heating and cooling; the diverse thermal responses were strikingly similar to those described in mammals. These data establish a functional map of thermoresponsive neurons in Drosophila larvae and provide a foundation for understanding mechanisms of thermoreception in both insects and mammals.
尽管感知温度的能力对许多生物体至关重要，但其潜在机制却知之甚少。我们使用钙指示剂黄色变色龙2.1和电生理记录，在黑腹果蝇幼虫中识别出热敏神经元，并检测了它们的生理反应。在头部，终末感觉器官神经元对降温≤1℃有活动增强反应，升温则降低其基础活动，不同的神经元单元表现出不同的反应模式。降温或升温均不影响背器官神经元。体壁神经元对升温和降温均表现出多种不同的反应模式；这些多样的热反应与哺乳动物中描述的极为相似。这些数据建立了果蝇幼虫热反应神经元的功能图谱，并为理解昆虫和哺乳动物的热感受机制提供了基础。
