Chen J, Jin X, Zhou L
Department of ENT, Renji Hospital, Shanghai Medical University, Shanghai 200001.
Lin Chuang Er Bi Yan Hou Ke Za Zhi. 1999 Jun;13(6):268-70.
To study the correlation between the expression level of Cyclin D1 and the laryngeal squamous cell carcinoma (LSCC).
35 LSCC cases testified pathologically were included in the study. The samples obtained from each tumor tissue and adjacent normal mucosa were detected to see the expression level of Cyclin D1 with the method of RT-PCR and light density half quantive analysis.
The expression level of Cyclin D1 was significant higher in almost all turmor tissues than in the adjacent normal mucosae (P < 0.05). Overexpressed carcinomas were more frequently associated with lymph node metastases (P < 0.05). A significant association between the expression level of Cyclin D1 and TNM was found. The higher T stage, the higher the expression level of Cyclin D1 (P < 0.01). The expression level of low histological grade carcinomas was lower than that of the high histological grade carcinomas, but the difference had no statistical significance. No association between overexpression of Cyclin D1 and sex, age was found too.
The result indicates that the expression level of Cyclin D1 in LSCC may be a useful marker of prognostic significance and it provide a molecular biology basis for treating the LSCC patients.
研究细胞周期蛋白D1（Cyclin D1）表达水平与喉鳞状细胞癌（LSCC）的相关性。
本研究纳入35例经病理证实的LSCC病例。采用逆转录聚合酶链反应（RT-PCR）和光密度半定量分析方法，检测各肿瘤组织及其相邻正常黏膜组织中Cyclin D1的表达水平。
几乎所有肿瘤组织中Cyclin D1的表达水平均显著高于相邻正常黏膜组织（P < 0.05）。Cyclin D1过表达的癌组织更易发生淋巴结转移（P < 0.05）。发现Cyclin D1表达水平与TNM分期之间存在显著关联。T分期越高，Cyclin D1表达水平越高（P < 0.01）。低组织学分级癌的Cyclin D1表达水平低于高组织学分级癌，但差异无统计学意义。Cyclin D1过表达与性别、年龄之间也无关联。
结果表明，LSCC中Cyclin D1的表达水平可能是一个具有预后意义的有用标志物，为LSCC患者的治疗提供了分子生物学依据。
