Wang Lingu, Duan Xinhu, Tang Xiabin, Shao Yuan, Zhao Ruimin, Qian Xiaoyun, Gao Xia
Department of Otolaryngology-Head and Neck Surgery, First Affiliated Hospital, Medical College of Xi' an Jiaotong University, Xi' an710061, China.
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2011 Nov;25(21):966-9.
To examine the expression and clinical significance of the Stat3 and Cyclin D1 in laryngeal squamous cell carcinoma (LSCC).
The expression of Stat3 and Cyclin D1 in LSCC tissue were detected by the RT-PCR and Western blot method respectively, and then the relationship between Stat3 and Cyclin D1 protein expression level and relevant clinical factors of LSCC was explored.
Compared to the normal larynx mucosa group, the expression level of Stat3 and Cyclin D1 was significantly higher in LSCC (P < 0.01). The high expression level of Stat3 and Cyclin D1 protein in LSCC was correlated with the clinical stage, tumor differentiation and lymph node metastases (P < 0.01). The expression level of Stat3 was positively correlated with that of Cyclin D1, The correlation coefficient(r) was 0.564 (P < 0.01) for the protein expression and 0.552 (P < 0.01) for the mRNA expression.
Stat3 signaling pathway may play an important role in the tumorigenesis and progression of LSCC. The expression level of Stat3 and its target gene Cyclin D1 can reflect the malignancy degree of LSCC.
检测信号转导与转录激活因子3（Stat3）和细胞周期蛋白D1（Cyclin D1）在喉鳞状细胞癌（LSCC）中的表达情况，并探讨其临床意义。
分别采用逆转录聚合酶链反应（RT-PCR）法和蛋白质免疫印迹法检测LSCC组织中Stat3和Cyclin D1的表达情况，分析Stat3和Cyclin D1蛋白表达水平与LSCC相关临床因素之间的关系。
与正常喉黏膜组相比，LSCC组织中Stat3和Cyclin D1的表达水平显著升高（P < 0.01）。LSCC中Stat3和Cyclin D1蛋白的高表达与临床分期、肿瘤分化程度及淋巴结转移相关（P < 0.01）。Stat3的表达水平与Cyclin D1呈正相关，蛋白表达的相关系数（r）为0.564（P < 0.01），mRNA表达的相关系数（r）为0.552（P < 0.01）。
Stat3信号通路可能在LSCC的发生发展中起重要作用。Stat3及其靶基因Cyclin D1的表达水平可反映LSCC的恶性程度。
