Qu J Q, Guan L R, Shulidan I, Zuo X P, Chai J J, Chen S B, Kawazu S, Katakura K, Matsumoto Y, Reed S G, Chang K P
Institute of Parasit Diseases, Chinese Academy of Preventive Medicine, Shanghai 200025.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2000;18(3):155-8.
To evaluate the diagnostic value of the recombinant antigen of 39 amino acid repeats encoded by a kinesin-like gene of Leishmania changasi (rK39) in serodiagnosis of visceral leishmaniasis (VL).
In Kashi, Xinjiang, 13 VL patients with splenomegaly and bone marrow aspirate culture positive were subjected to dipstick assay. A drop of whole blood or serum from patient was placed at the absorbing pad at the bottom of the dipstick. Flooding of the bottom protein with buffer allows serum proteins to migrate upwards, producing the positive band and Western blot analysis of rK39 subsequently performed with the sera collected.
The end-point titers of anti-rK39 antibodies of these sera were determined by ELISA and found to fall within the range of 10(-2) to 10(-4), which were consistent with the intensity of their reaction with rK39 in dipstick assay. The positive sera could also recognize the specific rK39 band as analyzed by Western blot analysis.
The rK39 dipstick assay is more rapid, specific, sensitive and less invasive than the conventional methods of diagnosis for VL in the areas of low endemicity.
评估杜氏利什曼原虫驱动蛋白样基因编码的39个氨基酸重复序列重组抗原（rK39）在内脏利什曼病（VL）血清学诊断中的价值。
在新疆喀什，对13例脾肿大且骨髓穿刺培养呈阳性的VL患者进行试纸条检测。将患者的一滴全血或血清置于试纸条底部的吸收垫上。用缓冲液冲洗底部蛋白质，使血清蛋白向上迁移，产生阳性条带，随后对收集的血清进行rK39的蛋白质印迹分析。
通过酶联免疫吸附测定（ELISA）测定这些血清中抗rK39抗体的终点滴度，发现其在10（-2）至10（-4）范围内，这与它们在试纸条检测中与rK39的反应强度一致。蛋白质印迹分析显示，阳性血清也能识别特定的rK39条带。
在低流行地区，rK39试纸条检测比传统的VL诊断方法更快速、特异、灵敏且侵入性更小。
