Zhao Y P, Tang H F, Jiang Y P, Wang Z Z, Yi Y H, Lei Q Y
Department of Pharmacy, Fourth Military Medical University, Xi'an 710032, China.
Yao Xue Xue Bao. 2001 Sep;36(9):660-3.
To study the triterpenoid saponins in the Chinese traditional medicine Eclipta prostrata L..
Column chromatography with silica gel and HPLC were employed for the isolation and purification. The molecular structures were determined on the basis of spectral analysis (IR, MS, 1HNMR, 13CNMR, HMQC and HMBC).
Two new triterpenoid saponins, named eclalbasaponins XI (4) and XII (5), were obtained and their structures were elucidated as 3-O-[beta-D-glucopyranosyl(1-->2)-beta-D-glucopyranosyl]-16 alpha-ethoxy-olean-12-ene-28-oic acid-28-O-beta-D-glucopyranoside and 3-O-[(2-O-sulfuryl-beta-D-glucopyranosyl) (1-->2)-beta-D-glucopyranosyl]-echinocystic acid-28-O-beta-D-glucopyranoside, respectively, along with three known saponins, eclalbasaponins II (1), I (2) and III (3).
Compounds 4 and 5 are new compounds, 1 and 5 induced morphological deformation of Pyricularia oryzae mycelia.
研究中药墨旱莲中的三萜皂苷。
采用硅胶柱色谱和高效液相色谱进行分离纯化。基于光谱分析（红外光谱、质谱、1H核磁共振、13C核磁共振、异核多量子相干谱和异核多键相关谱）确定分子结构。
获得了两种新的三萜皂苷，命名为墨旱莲皂苷XI（4）和XII（5），其结构分别被鉴定为3 - O - [β - D - 吡喃葡萄糖基（1→2）-β - D - 吡喃葡萄糖基]-16α - 乙氧基 - 齐墩果 - 12 - 烯 - 28 - 酸 - 28 - O - β - D - 吡喃葡萄糖苷和3 - O - [（2 - O - 磺酰基 - β - D - 吡喃葡萄糖基）（1→2）-β - D - 吡喃葡萄糖基]-刺囊酸 - 28 - O - β - D - 吡喃葡萄糖苷，同时还得到了三种已知皂苷，墨旱莲皂苷II（1）、I（2）和III（3）。
化合物4和5为新化合物，1和5可引起稻瘟病菌丝体形态变形。
