Sollepura Boregowda Rajini, Murali Nandhini, C Udayashankar Arakere, R Niranjana Siddapura, S Lund Ole, S Prakash Harischandra
Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysuru 570006, India.
Department of Plant and Environmental Science, University of Copenhagen, HøjbakkegårdAllé 13, 2630 Taastrup, Denmark.
Plants (Basel). 2019 Mar 22;8(3):72. doi: 10.3390/plants8030072.
Unscientific use of synthetic fungicides in plant disease management has environmental ramifications, such as disease resurgence and serious health problems due to their carcinogenicity. This has prompted the identification and development of eco-friendly greener alternatives. extract was evaluated for its antifungal activity in in vitro and in vivo against sorghum fungal pathogens and . The column purified methanolic extract of exhibited good antifungal activity against the target pathogens. The MIC was observed at 80 mg/mL for all tested pathogenic fungi, whereas MFC was 80 mg/mL for , 100 mg/mL for , and In vitro germination percentage was significantly high in seeds treated with extract (98%) over untreated control (91%). Significant disease protection of 95% was observed in greenhouse and 66% disease protection was noticed in field experiments. The efficacy of extract in field conditions was improved with the use of extract formulation. The profile of phytochemicals in methanol fractions was obtained by ultra-performance liquid chromatography (UPLC) mass spectroscopy. The [M-H] at / 313.3, / 797.9, and / 269.0 revealed the presence of wedelolactone, eclalbasaponin II, and apigenin, respectively. The H-nuclear magnetic resonance spectroscopy (¹H-NMR) chemical shift value supported the findings of the mass spectrometry. The results highlighted the possible use of methanolic extract as alternative to chemical fungicide in sorghum disease management.
在植物病害管理中不科学地使用合成杀菌剂会产生环境影响，例如由于其致癌性导致病害复发和严重的健康问题。这促使人们识别和开发环保型的更绿色替代物。对提取物进行了体外和体内抗高粱真菌病原体和 的抗真菌活性评估。柱纯化的 的甲醇提取物对目标病原体表现出良好的抗真菌活性。所有测试致病真菌的最低抑菌浓度（MIC）均为80 mg/mL，而 、 、 和 的最低杀菌浓度（MFC）分别为80 mg/mL、100 mg/mL、 和 。用 提取物处理的种子的体外发芽率（98%）显著高于未处理对照（91%）。在温室中观察到显著的病害防治效果为95%，在田间试验中观察到病害防治效果为66%。通过使用 提取物制剂提高了 提取物在田间条件下的功效。通过超高效液相色谱（UPLC）质谱获得了 甲醇馏分中的植物化学物质谱。在 / 313.3、 / 797.9和 / 269.0处的[M-H]分别表明存在蟛蜞菊内酯、刺蒺藜皂甙II和芹菜素。氢核磁共振光谱（¹H-NMR）化学位移值支持了质谱的结果。结果突出了 甲醇提取物在高粱病害管理中作为化学杀菌剂替代品的可能用途。
