[高效液相色谱法测定番荔枝科植物中的番荔枝内酯]
[Determination of annonaceous acetogenins in annonaceae plants by HPLC].
作者信息
Sun L, Yu J G, Li D Y, Li J, Yang X D, Yang S L
机构信息
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100094, China.
出版信息
Yao Xue Xue Bao. 2001 Sep;36(9):683-5.
AIM
To develop a method for analysis of antitumor annonaceous acetogenins in Annonaceae plants by HPLC.
METHODS
Squamostatin-B (1), squamocin (2) and annonin-VI (3) were used as standard substances. Chromatography column was a Rp-18; the mobile phase was methanol-water (90:10); the flow rate was 1.0 mL.min-1 and the detecting wavelength was 220 nm.
RESULTS
A linear range was obtained from 2.3 to 13.8 micrograms with a good correlation. The recoveries of (1), (2) and (3) were 100.3%, 100.3% and 100.0%, respectively.
CONCLUSION
This method was developed for the analysis of acetogenins by HPLC for the first time. The method is rapid, accurate and suitable for the analysis of the antitumor acetogenins in Annonaceae plants.
目的
建立一种用高效液相色谱法分析番荔枝科植物中抗肿瘤番荔枝内酯的方法。
方法
以鳞尾木素 -B（1）、鳞尾木素（2）和番荔枝宁 -VI（3）为标准品。色谱柱为Rp - 18；流动相为甲醇 - 水（90:10）；流速为1.0 mL·min⁻¹，检测波长为220 nm。
结果
得到了2.3至13.8微克的线性范围，相关性良好。（1）、（2）和（3）的回收率分别为100.3%、100.3%和100.0%。
结论
首次建立了用高效液相色谱法分析番荔枝内酯的方法。该方法快速、准确，适用于番荔枝科植物中抗肿瘤番荔枝内酯的分析。
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