来自皱盖乌芝的SCG（1,3-β-D-葡聚糖）在体外对DBA/2小鼠的γ-干扰素诱导作用。

IFN-gamma induction by SCG, 1,3-beta-D-glucan from Sparassis crispa, in DBA/2 mice in vitro.

作者信息

Harada Toshie, Miura Noriko N, Adachi Yoshiyuki, Nakajima Mitsuhiro, Yadomae Toshiro, Ohno Naohito

机构信息

Laboratory for Immunopharmacology of Microbial Products, School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.

出版信息

J Interferon Cytokine Res. 2002 Dec;22(12):1227-39. doi: 10.1089/10799900260475759.

DOI:10.1089/10799900260475759

PMID:12581496

Abstract

Sparassis crispa Fr. in an edible mushroom recently cultivable in Japan. A branched beta-glucan from S. crispa (SCG) is a major 6-branched 1,3-beta-D-glucan showing antitumor activity. In this study, we examined interferon-gamma (IFN-gamma) induction by SCG from splenocytes in DBA/2 mice in vitro. In the splenocytes derived from almost all inbred strains of mice except for DBA/1 and DBA/2 mice, IFN-gamma production was not induced by SCG. The breeder and genders of DBA/2 mice showed no influence on IFN-gamma induction by SCG. On the other hand, the magnitude of IFN-gamma induction was lower in young mice than in their older counterparts. IFN-gamma was induced by SCG in adherent splenocytes, but IFN-gamma production was most significantly increased by SCG in instances involving coexistence of adherent and nonadherent splenocytes. In fact, inhibition of cell-cell contact reduced IFN-gamma induction by SCG. In addition, interleukin-12 p70 (IL-12p70) was induced by SCG in DBA/2 mice. It was suggested that soluble factors and cell-cell contact mediate synergistic effects on SCG-induced IFN-gamma production.

摘要

皱盖乌芝是一种最近在日本可人工栽培的食用蘑菇。从皱盖乌芝中提取的一种分支β-葡聚糖（SCG）是一种主要的6分支1,3-β-D-葡聚糖，具有抗肿瘤活性。在本研究中，我们在体外检测了DBA/2小鼠脾细胞中SCG对干扰素-γ（IFN-γ）的诱导作用。除DBA/1和DBA/2小鼠外，几乎所有近交系小鼠的脾细胞中，SCG均未诱导IFN-γ的产生。DBA/2小鼠的饲养者和性别对SCG诱导IFN-γ的作用没有影响。另一方面，年轻小鼠中IFN-γ的诱导幅度低于老年小鼠。SCG在贴壁脾细胞中诱导IFN-γ，但在贴壁和非贴壁脾细胞共存的情况下，SCG使IFN-γ的产生增加最为显著。事实上，抑制细胞间接触可降低SCG对IFN-γ的诱导作用。此外，SCG在DBA/2小鼠中诱导白细胞介素-12 p70（IL-12p70）。提示可溶性因子和细胞间接触介导了对SCG诱导IFN-γ产生的协同作用。