Kim Yong-Woon, Wu Yuanzheng, Choi Moon-Hee, Shin Hyun-Jae, Li Jishun
Department of Biochemical and Polymer Engineering, Chosun University, Gwangju 61452, Korea.
Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250103, China.
J Fungi (Basel). 2020 Jun 25;6(2):92. doi: 10.3390/jof6020092.
This study aimed to investigate the elicitation effects of alginate oligosaccharides extracted from brown algae ( species) on β-glucan production in cauliflower mushroom (). Sodium alginate was refined from , , and , and characterized by proton nuclear magnetic resonance spectroscopy (H NMR), resulting mannuronic acid to guluronic acid (M/G) rationes from 0.64 to 1.38. Three oligosaccharide fractions, ethanol fraction (EF), solid fraction (SF), and liquid fraction (LF), were prepared by acid hydrolysis and analyzed by Fourier transform infrared (FT-IR) spectra and high-performance anion-exchange chromatography with a pulsed amperometric detector (HPAEC-PAD). The samples of resulted in the highest hydrolysate in SF and the lowest in LF, which was consistent with its highest M/G ratio. The SF of and LF of were chosen for elicitation on , yielding the highest β-glucan contents of 56.01 ± 3.45% and 59.74 ± 4.49% in the stalk, respectively. Total polyphenol content (TPC) and antioxidant activities (2,2'-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging and Superoxide dismutase (SOD)-like activity) of aqueous extracts of were greatly stimulated by alginate elicitation. These results demonstrate that alginate oligosaccharides extracted from brown algae may be useful as an elicitor to enhance the nutritional value of mushrooms.
本研究旨在探讨从褐藻（物种）中提取的海藻酸钠寡糖对花椰菜蘑菇中β-葡聚糖产生的诱导作用。海藻酸钠由[具体褐藻种类1]、[具体褐藻种类2]和[具体褐藻种类3]精制而成，并通过质子核磁共振光谱（H NMR）进行表征，得到的甘露糖醛酸与古洛糖醛酸（M/G）比例为0.64至1.38。通过酸水解制备了三个寡糖级分，即乙醇级分（EF）、固体级分（SF）和液体级分（LF），并通过傅里叶变换红外（FT-IR）光谱和带脉冲安培检测器的高效阴离子交换色谱（HPAEC-PAD）进行分析。[具体褐藻种类]的样品在SF中产生的水解产物最高，在LF中最低，这与其最高的M/G比例一致。选择[具体褐藻种类]的SF和[具体褐藻种类]的LF对[花椰菜蘑菇]进行诱导，在菌柄中分别产生了最高的β-葡聚糖含量，为56.01±3.45%和59.74±4.49%。海藻酸钠诱导极大地刺激了[花椰菜蘑菇]水提取物的总多酚含量（TPC）和抗氧化活性（2,2'-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）（ABTS）自由基清除和超氧化物歧化酶（SOD）样活性）。这些结果表明，从褐藻中提取的海藻酸钠寡糖可能作为一种诱导剂来提高蘑菇的营养价值。
