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新杂交花椰菜蘑菇(Sparassis latifolia)的形态和遗传特征。

Morphological and genetic characteristics of newly crossbred cauliflower mushroom (Sparassis latifolia).

机构信息

Division of Wood Chemistry and Microbiology, Korea Forest Research Institute, Seoul, 130-712, Republic of Korea.

出版信息

J Microbiol. 2013 Oct;51(5):552-7. doi: 10.1007/s12275-013-2666-z. Epub 2013 Jun 25.

DOI:10.1007/s12275-013-2666-z
PMID:23800950
Abstract

Cauliflower mushroom (Sparassis latifolia or S. crispa) is popular for food and medicine. Importance of new varieties of Sparassis was raised and studied widely by protection system of UPOV. In this study, 10 crossbred strains of Sparassis latifolia that specifically expressed distinctive features during basidiocarp formation and mycelium growth were applied to sawdust medium inoculated with S. latifolia mycelia. The 10 crossbred strains were divided into 3 groups on the basis of morphological (size of marginal wave and basidiocarp color) and genetic characteristics. Each phenotype of the parent and crossbred strains represented 3 marginal wave-sizes (large, medium, and small) and 3 color notations (NN155D, 163C, and 8D). Our result suggests that morphological characteristics of cauliflower mushroom can be affected by various environmental and genetic stimuli under artificial conditions such as crossbreed. Also this research showed genetic differences among breeding isolates and their morphological characteristics were correlated with the molecular data within parent and crossed strain.

摘要

菜花蘑(Sparassis latifolia 或 S. crispa)既是美味佳肴,又是常用药材。因其重要的经济价值,UPOV 保护体系对其新品种广泛重视并进行了深入研究。本研究以特定形态(菌盖边缘波状和菌盖颜色)和遗传特征为基础,将 10 株菜花蘑杂交菌株应用于木屑培养基上接种的 Sparassis latifolia 菌丝。根据形态(菌盖边缘波状大小和菌盖颜色)和遗传特征,将 10 株杂交菌株分为 3 组。每组亲本和杂交菌株的表型代表 3 种菌盖边缘波状大小(大、中、小)和 3 种颜色标记(NN155D、163C 和 8D)。本研究表明,在人工条件下,如杂交,菜花蘑的形态特征可能会受到各种环境和遗传刺激的影响。此外,该研究还表明,在育种种群之间存在遗传差异,其形态特征与亲本和杂交菌株的分子数据相关。

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本文引用的文献

1
Phylogenetic relationships of Korean Sparassis latifolia based on morphological and ITS rDNA characteristics.基于形态学和 ITS rDNA 特征的韩国裂盖马鞍菌的系统发育关系。
J Microbiol. 2013 Feb;51(1):43-8. doi: 10.1007/s12275-013-2503-4. Epub 2013 Mar 2.
2
MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods.MEGA5:用于最大似然法、进化距离法和最大简约法的分子进化遗传学分析。
Mol Biol Evol. 2011 Oct;28(10):2731-9. doi: 10.1093/molbev/msr121. Epub 2011 May 4.
3
Phylogenetic relationships of Sparassis inferred from nuclear and mitochondrial ribosomal DNA and RNA polymerase sequences.
从核和线粒体核糖体 DNA 和 RNA 聚合酶序列推断的 Sparassis 系统发育关系。
Mycologia. 2004 Sep-Oct;96(5):1015-29.
4
Sparassis cystidiosa sp. nov. from Thailand is described using morphological and molecular data.泰国的裂褶菌新种 Sparassis cystidiosa sp. nov. 是根据形态学和分子数据描述的。
Mycologia. 2004 Sep-Oct;96(5):1010-4.
5
NMR characterization of the structure of a beta-(1-->3)-D-glucan isolate from cultured fruit bodies of Sparassis crispa.皱盖乌芝培养子实体中β-(1→3)-D-葡聚糖分离物结构的核磁共振表征
Carbohydr Res. 2007 Dec 10;342(17):2611-8. doi: 10.1016/j.carres.2007.08.016. Epub 2007 Aug 31.
6
Phylogeny and a new species of Sparassis (Polyporales, Basidiomycota): evidence from mitochondrial atp6, nuclear rDNA and rpb2 genes.绣球菌属(多孔菌目,担子菌门)的系统发育及一个新物种:来自线粒体atp6、核rDNA和rpb2基因的证据
Mycologia. 2006 Jul-Aug;98(4):584-92. doi: 10.3852/mycologia.98.4.584.
7
IFN-gamma induction by SCG, 1,3-beta-D-glucan from Sparassis crispa, in DBA/2 mice in vitro.来自皱盖乌芝的SCG(1,3-β-D-葡聚糖)在体外对DBA/2小鼠的γ-干扰素诱导作用。
J Interferon Cytokine Res. 2002 Dec;22(12):1227-39. doi: 10.1089/10799900260475759.
8
DNA polymorphisms amplified by arbitrary primers are useful as genetic markers.用任意引物扩增的DNA多态性可作为遗传标记。
Nucleic Acids Res. 1990 Nov 25;18(22):6531-5. doi: 10.1093/nar/18.22.6531.