水稻品种中部32中赋予对叶瘟部分抗性的QTL（数量性状基因座）定位。
Mapping of the QTL (quantitative trait locus) conferring partial resistance to leaf blast in rice cultivar Chubu 32.
作者信息
Zenbayashi K., Ashizawa T., Tani T., Koizumi S.
机构信息
National Agricultural Research Center for Tohoku Region, Yotsuya, Omagari, Akita 014-0102, Japan.
出版信息
Theor Appl Genet. 2002 Mar;104(4):547-552. doi: 10.1007/s00122-001-0779-y.
The rice cultivar Chubu 32 possesses a high level of partial resistance to leaf blast. The number and chromosomal location of genes conferring this resistance were detected by restriction fragment length polymorphism (RFLP) linkage mapping and quantitative trait locus (QTL) analysis. For the mapping, 149 F(3) lines derived from the cross between rice cultivar Norin 29, with a low level of partial resistance, and Chubu 32 were used, and their partial resistance to leaf blast was assessed in upland nurseries. A linkage map covering six chromosomes and consisting of 36 RFLP markers was constructed. In the map, only one significant QTL (LOD>2.0) for partial resistance was detected on chromosome 11. This QTL explained 45.6% of the phenotypic variation. The segregation ratio of the F3 lines was 3:1 for partial resistance to susceptibility. These results suggest that the partial resistance in Chubu 32 is controlled by a major gene.
水稻品种中部32对叶瘟具有较高水平的部分抗性。通过限制性片段长度多态性（RFLP）连锁图谱分析和数量性状位点（QTL）分析，检测了赋予这种抗性的基因数量和染色体定位。为了进行图谱构建，使用了由水稻品种Norin 29（部分抗性水平较低）与中部32杂交产生的149个F(3)株系，并在旱地苗圃中评估了它们对叶瘟的部分抗性。构建了一个覆盖6条染色体、由36个RFLP标记组成的连锁图谱。在该图谱中，仅在第11号染色体上检测到一个对部分抗性有显著影响的QTL（LOD>2.0）。该QTL解释了45.6%的表型变异。F3株系对部分抗性与易感性的分离比为3:1。这些结果表明，中部32的部分抗性受一个主基因控制。
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