Vreim C E, Staub N C
Am J Physiol. 1976 Feb;230(2):376-9. doi: 10.1152/ajplegacy.1976.230.2.376.
In 11 anesthetized dogs with acute alloxan-induced pulmonary edema, we measured the protein composition of 1-mul samples of plasma, free interstitial fluid, alveolar fluid, and airway fluid. We obtained plasma and airway fluid at regular intervals as edema developed. We sampled alveolar fluid by pleural micropuncture in the unfrozen, excised lung and free interstitial fluid from perivascular cuffs in the frozen, excised lung. The average (+/- 1 SD) total protein concentration of plasma was 4.9 +/- 0.6, airway fluid 4.4 +/- 0.7, free interstitial fluid 4.9 +/- 0.7, and alveolar fluid 5.2 +/- 0.8 g/100 ml. The average fractions of albumin were 0.42 +/- 0.05, 0.50 +/- 0.05, 0.49 +/- 0.06, and 0.49 +/- 0.07, respectively. By paired analysis, the protein concentration of interstitial fluid was not significantly different from alveolar fluid. The protein concentration of airway fluid was significantly less than that in interstitial and alveolar fluid. The albumin fraction of the three lung fluids was identical but significantly different from plasma. We conclude that in alloxan-induced pulmonary edema the lung fluids contain high concentrations of protein and the alveolar epithelial membrane becomes freely permeable to protein molecules.
在11只经麻醉、用四氧嘧啶诱导产生急性肺水肿的犬中，我们测量了血浆、游离间质液、肺泡液和气道液1微升样本的蛋白质组成。随着水肿的发展，我们定期获取血浆和气道液。我们通过在未冷冻的离体肺组织上进行胸膜微穿刺来采集肺泡液，并通过在冷冻的离体肺组织的血管周围袖套处采集游离间质液。血浆的平均（±1标准差）总蛋白浓度为4.9±0.6、气道液为4.4±0.7、游离间质液为4.9±0.7、肺泡液为5.2±0.8 g/100 ml。白蛋白的平均比例分别为0.42±0.05、0.50±0.05、0.49±0.06和0.49±0.07。通过配对分析，间质液的蛋白浓度与肺泡液无显著差异。气道液的蛋白浓度显著低于间质液和肺泡液。三种肺液的白蛋白比例相同，但与血浆有显著差异。我们得出结论，在四氧嘧啶诱导的肺水肿中，肺液含有高浓度的蛋白质，并且肺泡上皮膜对蛋白质分子变得自由通透。
