Dajani A S, Tom M C, Law D J
Antimicrob Agents Chemother. 1976 Jan;9(1):81-8. doi: 10.1128/AAC.9.1.81.
Bacteriocin-like activities were detected in 78% of 120 alpha-hemolytic streptococcal isolates. Inhibitory substances from three such isolates (one Streptococcus sanguis strain and two S. mitis strains) were investigated further and termed viridins (A, B, and C). The viridins were unique among bacteriocins of gram-positive bacteria in that they inhibited many gram-negative bacteria in addition to inhibition of a variety of gram-positive organisms. Viridins were obtained in a cell-free state only after mechanical disruption of bacteriocinogenic cells but could not be isolated from supernatant fluids of cultures of such bacteria or from freeze-thaw liquor of agar on which the bacteria had been grown. Viridin B could be partially purified by ammonium sulfate precipitation and by gel filtration on a Sephadex G-100 column. This bacteriocin had some unusual properties including heat lability, a narrow pH range of activity, and lack of adsorptive capacity to susceptible bacteria. Although viridin B was bactericidal to a Neisseria sicca strain, it was only bacteriostatic against a coagulase-negative staphylococcus.
在120株α-溶血性链球菌分离株中，78%检测到类细菌素活性。对其中三株分离株（一株血链球菌和两株缓症链球菌）产生的抑制物质进行了进一步研究，并将其命名为绿菌素（A、B和C）。绿菌素在革兰氏阳性菌的细菌素中独具特色，因为它们除了能抑制多种革兰氏阳性菌外，还能抑制许多革兰氏阴性菌。绿菌素只有在对产生细菌素的细胞进行机械破碎后才能以无细胞状态获得，但无法从这类细菌培养物的上清液或细菌生长所用琼脂的冻融液中分离得到。绿菌素B可通过硫酸铵沉淀和在Sephadex G - 100柱上进行凝胶过滤进行部分纯化。这种细菌素具有一些不寻常的特性，包括热稳定性差、活性pH范围窄以及对敏感细菌缺乏吸附能力。虽然绿菌素B对干燥奈瑟菌菌株具有杀菌作用，但对凝固酶阴性葡萄球菌仅具有抑菌作用。
