Tagg J R, Read R S, McGiven A R
Antimicrob Agents Chemother. 1973 Sep;4(3):214-21. doi: 10.1128/AAC.4.3.214.
The production of bacteriocin-like inhibition by certain strains of group A streptococci was demonstrated during the growth of these organisms on solid nutrient media. Active bacteriocin could not be recovered from broth cultures, possibly because of its inactivation by streptococcal proteinase. The bacteriocin, streptocin A, produced by Streptococcus pyogenes strain FF-22 was partially purified by chemical precipitation and chromatography on carboxymethyl-cellulose. Streptocin A inhibited the growth of various gram-positive organisms but none of a wide range of different gram-negative strains. The bacteriocin was inactivated by proteolytic enzymes and, although labile in alkali, it was extremely stable to heating in mild acids.
某些A组链球菌菌株在固体营养培养基上生长期间可产生类细菌素抑制作用。在肉汤培养物中无法回收活性细菌素，这可能是因为它被链球菌蛋白酶灭活。化脓性链球菌FF - 22菌株产生的细菌素链菌素A通过化学沉淀和羧甲基纤维素柱色谱法进行了部分纯化。链菌素A抑制各种革兰氏阳性菌的生长，但对多种不同的革兰氏阴性菌株均无抑制作用。该细菌素可被蛋白水解酶灭活，虽然在碱性条件下不稳定，但在弱酸中加热时却极其稳定。
