噬菌体的形态学同质性及其生物活性。

Morphologic homogeneity of bacteriophages and their biological activity.

作者信息

Krzywy T, Kucharewicz-Krukowska A, Slopek S

出版信息

Arch Immunol Ther Exp (Warsz). 1976;24(1):29-37.

Abstract

Lytic activity of morphologically homogeneous phage lysates, obtained from lysates containing morphologically inhomogeneous virions, was studied. Five series of phage lysates found to contain morphologically inhomogeneous virions were investigated. By selecting suitable hosts from each series, two or three lines were obtained which reproduced morphologically homogeneous virions. Morphologic homogeneity of these phage lines was determined by electron microscopy. In the course of this study it was found that phages with different morphologic appearance can produce similar plaques. Hence, appearance of plaques is not an adequate criterion of homogeneity of the phage. The isolated phages differed in biological activity from the original morphologically inhomogeneous bacteriophage. It was concluded that morphology is an important, and the most reliable at present, criterion of homogeneity of bacteriophages.

摘要

对从含有形态不均一病毒粒子的裂解物中获得的形态均一噬菌体裂解物的裂解活性进行了研究。对发现含有形态不均一病毒粒子的五组噬菌体裂解物进行了调查。通过从每组中选择合适的宿主，获得了两到三个产生形态均一病毒粒子的品系。这些噬菌体品系的形态均一性通过电子显微镜确定。在这项研究过程中发现，具有不同形态外观的噬菌体可以产生相似的噬菌斑。因此，噬菌斑的外观不是噬菌体均一性的充分标准。分离出的噬菌体在生物学活性上与原始的形态不均一噬菌体不同。得出的结论是，形态是噬菌体均一性的一个重要标准，并且是目前最可靠的标准。