Ha Un-Hwan, Wang Yanping, Jin Shouguang
Department of Molecular Genetics and Microbiology, University of Florida, Gainesville, Florida 32610, USA.
Infect Immun. 2003 Mar;71(3):1590-5. doi: 10.1128/IAI.71.3.1590-1595.2003.
DsbA is a periplasmic thiol:disulfide oxidoreductase which contributes to the process of protein folding by catalyzing the formation of disulfide bonds. In this study, we demonstrate that the dsbA gene is required for the expression of the type III secretion system under low-calcium inducing conditions, intracellular survival of P. aeruginosa upon infection of HeLa cells, and twitching motility. The diverse phenotypes of the dsbA mutant are likely due to its defect in the folding of proteins that are involved in various biological processes, such as signal sensing, protein secretion, and defense against host clearing. In light of its effect on various virulence factors, DsbA could be an important target for the control of P. aeruginosa infections.
DsbA是一种周质硫醇：二硫键氧化还原酶，它通过催化二硫键的形成促进蛋白质折叠过程。在本研究中，我们证明dsbA基因是III型分泌系统在低钙诱导条件下表达、铜绿假单胞菌感染HeLa细胞后的细胞内存活以及颤动运动所必需的。dsbA突变体的多种表型可能是由于其在参与各种生物学过程（如信号传感、蛋白质分泌和抵御宿主清除）的蛋白质折叠方面存在缺陷。鉴于其对各种毒力因子的影响，DsbA可能是控制铜绿假单胞菌感染的重要靶点。
