Ballesteros J, Alvarez J B, Giménez M J, Ramírez M C, Cabrera A, Martín A
Departamento de Agronomía y Mejora Genética Vegetal, Instituto de Agricultura Sostenible, Consejo Superior de Investigaciones Científicas, Apdo. 4084, E-14080 Córdoba, Spain.
Theor Appl Genet. 2003 Feb;106(4):644-8. doi: 10.1007/s00122-002-1110-2. Epub 2002 Oct 19.
The uses of hexaploid tritordeum as a crop for human consumption require improvement of its bread-making quality. For this purpose chromosome 1D of bread wheat with the Glu-D1 allele encoding for high-molecular-weight glutenin subunits Dx5+Dy10 was introgressed into tritordeum. Different primary tritordeums were crossed with wheats carrying subunits Dx5+Dy10. The hybrids were backcrossed to tritordeum and seeds for the next backcross (or selfing) were selected for the presence of chromosome 1D using SDS-PAGE. Forty two chromosome plants carrying subunits Dx5+Dy10 were obtained after two backcrosses and selfing. Chromosome characterization of these plants using fluorescence in situ hybridisation (FISH) proved that either chromosome substitution 1H(ch)/1D or 1A/1D had been obtained. A homozygous plant with a translocation of the entire 1DL arm to 1H(ch)S was also obtained. The complete chromosome substitution lines have better agronomic characteristics than the lines with translocations.
将六倍体黑小麦用作人类食用作物，需要改善其制面包品质。为此，将携带编码高分子量谷蛋白亚基Dx5 + Dy10的Glu - D1等位基因的面包小麦1D染色体渗入黑小麦。将不同的初级黑小麦与携带亚基Dx5 + Dy10的小麦杂交。杂种与黑小麦回交，并使用SDS - PAGE选择用于下一次回交（或自交）的种子中是否存在1D染色体。经过两次回交和自交后，获得了42株携带亚基Dx5 + Dy10的染色体植株。使用荧光原位杂交（FISH）对这些植株进行染色体鉴定，结果表明获得了1H（ch）/1D或1A/1D染色体替代植株。还获得了一个将整个1DL臂易位到1H（ch）S的纯合植株。完整的染色体替代系比易位系具有更好的农艺性状。
