Barro F, Martín A, Cabrera A
Instituto de Agricultura Sostenible, CSIC, Apdo 4084, 14080 Córdoba, Spain.
Chromosome Res. 2003;11(6):565-72. doi: 10.1023/a:1024948700938.
Plants from two transgenic lines of tritordeum (an amphiploid between Triticum turgidum cv. durum and Hordeumn chilense) have been analyzed by fluorescence in-situ hybridization (FISH) to characterize the transgene integration sites and chromosome rearrangements. Transgenic lines were transformed in two different events with the genes encoding for the high-molecular-weight glutenin subunits (HMW-GS), 1Ax1 and/or 1Dx5. Three integration sites and four translocations were detected. All three integration sites were located on chromosome segments of Hordeum chilense translocated into wheat chromosomes. No translocations from wheat into H. chilense chromosomes were observed. Both HMW-GS transgenes were expressed at high levels in the endosperm of transgenic plants. The analysis by FISH of transgenic plants allowed the early detection of homozygous and heterozygous plants. The consequences and implications of translocations on breeding are discussed.
对来自两个黑小麦转基因株系（硬粒小麦栽培品种与智利大麦的双二倍体）的植株进行了荧光原位杂交（FISH）分析，以表征转基因整合位点和染色体重排情况。转基因株系通过两个不同事件用编码高分子量谷蛋白亚基（HMW-GS）1Ax1和/或1Dx5的基因进行转化。检测到三个整合位点和四个易位。所有三个整合位点均位于易位到小麦染色体上的智利大麦染色体片段上。未观察到从小麦到智利大麦染色体的易位。两个HMW-GS转基因在转基因植物的胚乳中均高水平表达。通过FISH对转基因植物进行分析可早期检测纯合和杂合植物。讨论了易位对育种的影响和意义。
