Veazey Ronald S, Marx Preston A, Lackner Andrew A
Tulane National Primate Research Center, Health Sciences Center, Tulane University, 18703 Three Rivers Road, Covington, LA 70433, USA.
J Infect Dis. 2003 Mar 1;187(5):769-76. doi: 10.1086/368386. Epub 2003 Feb 24.
Worldwide, the majority of human immunodeficiency virus-1 cases occur through heterosexual transmission, yet little is known regarding the phenotype of CD4(+) T cells in the vaginal mucosa. In the present study, lymphocytes were compared from the lymph nodes, blood, and vagina from uninfected and simian immunodeficiency virus (SIV)-infected macaques. In mature female macaques, 54%-67% of the vaginal CD4(+) T cells expressed C chemokine receptor 5 (CCR5), whereas 84%-99% coexpressed CXC chemokine receptor 4. In contrast, only 4.4%-14.8% of peripheral blood and 2.4%-13% of lymph-node CD4(+) T cells coexpressed CCR5. Moreover, CCR5 mean channel fluorescence was significantly higher on CD4 cells from the vagina, compared with those from blood. In macaques intravenously infected with SIV, rapid depletion of CD4(+) T cells was observed in the vagina, particularly among the CCR5(+)CD4(+) subset. This demonstrates that large numbers of CD4(+) T cells expressing high levels of CCR5 reside within the vagina and that these cells are preferentially targeted for elimination by SIV infection.
在全球范围内，大多数人类免疫缺陷病毒1型病例是通过异性传播发生的，然而对于阴道黏膜中CD4(+) T细胞的表型却知之甚少。在本研究中，对未感染和感染猿猴免疫缺陷病毒（SIV）的猕猴的淋巴结、血液和阴道中的淋巴细胞进行了比较。在成年雌性猕猴中，54%-67%的阴道CD4(+) T细胞表达C趋化因子受体5（CCR5），而84%-99%的细胞共表达CXC趋化因子受体4。相比之下，外周血中只有4.4%-14.8%的CD4(+) T细胞和淋巴结中2.4%-13%的CD4(+) T细胞共表达CCR5。此外，与血液中的CD4细胞相比，阴道CD4细胞上的CCR5平均通道荧光显著更高。在静脉感染SIV的猕猴中，观察到阴道内CD4(+) T细胞迅速耗竭，尤其是在CCR5(+)CD4(+)亚群中。这表明大量表达高水平CCR5的CD4(+) T细胞存在于阴道内，并且这些细胞是SIV感染优先靶向清除的对象。
