Védrine Christophe, Leclerc Jean-Claude, Durrieu Claude, Tran-Minh Canh
Ecole Nationale Supérieure des Mines, SPIN/PC2M/Enzyme engineering, 158, Cours Fauriel, 42023 Saint-Etienne cedex 2, France.
Biosens Bioelectron. 2003 Apr;18(4):457-63. doi: 10.1016/s0956-5663(02)00157-4.
An optical biosensor was designed for determination of herbicides as aquatic contaminants. Detection was obtained with immobilised Chlorella vulgaris microalgae entrapped on a quartz microfibre filter and placed in a five-membrane-home-made-flow cell. The algal chlorophyll fluorescence modified by the presence of herbicides was collected at the tip of an optical fibre bundle and sent to a fluorimeter. A continuous culture was set up to produce algal cells in reproducible conditions for measurement optimisation. Effects of flow rate, algal density, temperature, and pH on the biosensor response to atrazine were studied. Reversibility and detection limits were determined for DNOC and atrazine, simazine, isoproturon, diuron. Detection of photosystem II (PSII) herbicides was achieved at sub-ppb concentration level.
设计了一种用于测定作为水生污染物的除草剂的光学生物传感器。通过将固定化的普通小球藻微藻截留在石英微纤维滤膜上并置于自制的五膜流通池中进行检测。由除草剂的存在而改变的藻类叶绿素荧光在光纤束的尖端收集并发送到荧光计。建立了连续培养以在可重复的条件下产生藻类细胞以优化测量。研究了流速、藻类密度、温度和pH对生物传感器对阿特拉津响应的影响。测定了DNOC、阿特拉津、西玛津、异丙隆、敌草隆的可逆性和检测限。在亚ppb浓度水平实现了对光系统II（PSII）除草剂的检测。
