Université de Lyon, Université Claude Bernard Lyon 1, CNRS, ENTPE, UMR5023 LEHNA, F-69518 Vaulx-en-Velin, France; Université de Lyon, INSA-Lyon, Laboratoire DEEP, EA7429, Bât. S. Carnot, 9 rue de la Physique, F-69621 Villeurbanne, France.
Université de Lyon, Université Claude Bernard Lyon 1, CNRS, ENTPE, UMR5023 LEHNA, F-69518 Vaulx-en-Velin, France.
Biosens Bioelectron. 2018 Oct 15;117:669-677. doi: 10.1016/j.bios.2018.07.005. Epub 2018 Jul 5.
A critical need exists to develop rapid, in situ, and real-time tools to monitor the impact of pollution discharge toxicity on aquatic ecosystems. The present paper deals with the development of a novel, simple-to-use, low-cost, portable, and user-friendly algal biosensor. In this study, a complete and autonomous portable fluorimeter was developed to assess the A-chlorophyll fluorescence of microalgae, inserted by capillarity into low-cost and disposable xurography-based microfluidic chips. Three microalgae populations were used to develop the biosensor: Chlorella vulgaris, Pseudokirchneriella subcapitata, and Chlamydomonas reinhardtii. Biosensor feasibility and sensitivity parameters, such as algal concentration and light intensity, were optimized beforehand to calibrate the biosensor sensitivity with Diuron, a pesticide known to be very toxic for microalgae. Finally, the biosensor was employed in 10 aqueous urban polluted samples (7 urban wet-weather discharges and 3 wastewater) in order to prove its reliability, reproducibility, and performance in the detection of toxic discharges in the field.
目前当务之急是开发快速、原位和实时的工具，以监测污染排放毒性对水生生态系统的影响。本文介绍了一种新型的、易于使用的、低成本的、便携式的和用户友好的藻类生物传感器的开发。在这项研究中，开发了一种完整的、自主的便携式荧光计，用于评估微藻的 A-叶绿素荧光，通过毛细作用插入低成本和一次性的基于 xurography 的微流控芯片中。使用三种微藻种群来开发生物传感器：普通小球藻、拟卡德利亚藻和莱茵衣藻。在优化生物传感器的可行性和灵敏度参数之前，例如藻类浓度和光强度，以用农药敌草隆对生物传感器的灵敏度进行校准，敌草隆已知对微藻具有很强的毒性。最后，该生物传感器被用于 10 个城市污染水样（7 个城市雨水和 3 个污水）中，以证明其在现场检测有毒排放物的可靠性、重现性和性能。
