Yoshimura-Uchiyama Chitose, Yamaguchi Masao, Nagase Hiroyuki, Fujisawa Takao, Ra Chisei, Matsushima Kouji, Iwata Tsutomu, Igarashi Takashi, Yamamoto Kazuhiko, Hirai Koichi
Department of Allergy and Rheumatology, University of Tokyo, Graduate School of Medicine, Japan.
Biochem Biophys Res Commun. 2003 Mar 7;302(2):201-6. doi: 10.1016/s0006-291x(03)00153-0.
IL-3, IL-5, and GM-CSF exert various overlapping functions in basophils. We investigated the receptor expression profiles and concentration-dependent effects of IL-3, IL-5, and GM-CSF on several basophil functions in comparison with their effects on eosinophils. The order of the receptor expression levels was IL-3Ralpha>IL-5Ralpha>GM-CSFRalpha in basophils and IL-5Ralpha>or=GM-CSFRalpha>IL-3Ralpha in eosinophils. Compared with eosinophils, basophils expressed a much higher level of IL-3Ralpha and similar levels of IL-5Ralpha and GM-CSFRalpha. The order of potency was IL-3>IL-5=GM-CSF for degranulation, survival, and CD11b expression in basophils, and IL-5=GM-CSF>or=IL-3 for survival and CD11b expression in eosinophils. However, IL-3 induced CD69 expression preferentially in basophils. Our results indicate that IL-3 is the most potent activator of human basophils, and that the rank order of potency of hemopoietic growth factors virtually corresponded to their receptor expression levels in both cell types.
白细胞介素-3（IL-3）、白细胞介素-5（IL-5）和粒细胞-巨噬细胞集落刺激因子（GM-CSF）在嗜碱性粒细胞中发挥多种重叠功能。我们研究了IL-3、IL-5和GM-CSF的受体表达谱及其对嗜碱性粒细胞多种功能的浓度依赖性效应，并与它们对嗜酸性粒细胞的效应进行了比较。嗜碱性粒细胞中受体表达水平的顺序为IL-3Rα>IL-5Rα>GM-CSFRα，而嗜酸性粒细胞中为IL-5Rα≥GM-CSFRα>IL-3Rα。与嗜酸性粒细胞相比，嗜碱性粒细胞表达的IL-3Rα水平高得多，而IL-5Rα和GM-CSFRα水平相似。在嗜碱性粒细胞中，脱颗粒、存活和CD11b表达的效力顺序为IL-3>IL-5 = GM-CSF，而在嗜酸性粒细胞中，存活和CD11b表达的效力顺序为IL-5 = GM-CSF≥IL-3。然而，IL-3优先诱导嗜碱性粒细胞中CD69的表达。我们的结果表明，IL-3是人类嗜碱性粒细胞最有效的激活剂，造血生长因子的效力等级顺序实际上与两种细胞类型中它们的受体表达水平相对应。
