Suit H D, Sedlacek R, Wagner M, Orsi L, Silobrcic V, Rothman K J
Cancer Res. 1976 Apr;36(4):1305-14.
A methylcholanthrene-induced fibrosarcoma of C3H mice has been used as 5th- to 6th-generation syngeneic transplant in an investigation of the role of Corynebacterium parvum as an adjuvant to the therapeutic effect of local irradiation of established tumors. The most effective route for administration of C. parvum in this tumor system was i.v., and the greatest effect of the C. parvum-mediated tumor graft rejection was observed for tumor growing intracutaneously or s.c. An intermediate level of effectiveness was obtained for tumor growing i.m. The combined C. parvum and local irradiation studies were performed using tumors growing in the leg muscle and measuring 8 mm in diameter at the time of local irradiation. Several routes of administration of C. parvum, dose levels of C. parvum, and time relationships between administration of C. parvum and irradiation were investigated. The outstanding finding was that very low doses of radiation were quite effective when administered to tumors growing in animals pretreated with C. parvum. This was true for radiation administered as a single or fractionated dose (10 equal doses spread over 18 days). For single-dose irradiation the effect was relatively dose independent over the range of 200 to 3000 rads. Some indication was obtained that local irradiation may impair the tumor graft rejection reaction. No evidence was obtained of an enhanced growth of tumor. Regression of tumor following irradiation was not modified by pretreatment with C. parvum. The mice that were unsuccessfully treated by radiation and C. parvum had a lower incidence of metastatic disease in the lung than did the mice treated unsuccessfully with radiation alone.
在一项关于短小棒状杆菌作为已建立肿瘤局部照射治疗效果辅助剂作用的研究中，将甲基胆蒽诱导的C3H小鼠纤维肉瘤用作第5至6代同基因移植瘤。在该肿瘤系统中，短小棒状杆菌最有效的给药途径是静脉注射，对于皮内或皮下生长的肿瘤，观察到短小棒状杆菌介导的肿瘤移植排斥作用最大。对于肌肉内生长的肿瘤，获得了中等程度的效果。联合短小棒状杆菌和局部照射的研究是使用腿部肌肉中生长的肿瘤进行的，在局部照射时肿瘤直径为8毫米。研究了短小棒状杆菌的几种给药途径、短小棒状杆菌的剂量水平以及短小棒状杆菌给药与照射之间的时间关系。突出的发现是，当对用短小棒状杆菌预处理的动物体内生长的肿瘤给予非常低剂量的辐射时，效果相当显著。对于单次或分次剂量（在18天内分10等份给予）的辐射都是如此。对于单次剂量照射，在200至3000拉德的范围内，效果相对与剂量无关。有迹象表明局部照射可能会损害肿瘤移植排斥反应。没有获得肿瘤生长增强的证据。照射后肿瘤的消退并未因短小棒状杆菌预处理而改变。经辐射和短小棒状杆菌治疗未成功的小鼠肺部转移疾病的发生率低于仅接受辐射治疗未成功的小鼠。
