Jensen Sigmund, Samuelsen Ole B, Andersen Kari, Torkildsen Lise, Lambert Christophe, Choquet Gwénaëlle, Paillard Christine, Bergh Oivind
Department of Aquaculture, Institute of Marine Research, PO Box 1870 Nordnes, 5817 Bergen, Norway.
Dis Aquat Organ. 2003 Jan 22;53(1):25-31. doi: 10.3354/dao053025.
Two vibrio bacteria pathogenic to the corkwing wrasse Symphodus melops were isolated. Vibriosis-inducing strain LP1 was isolated as the dominanting bacterium in kidney samples of dead and moribund wrasse from a population suffering vibriosis and high daily mortality in 1998 on the Norwegian west coast. The other vibriosis-inducing strain, LP2, was isolated from wrasse captured the following year. Re-infection experiments have confirmed that these strains cause vibriosis in corkwing wrasse. Both strains were typical vibrios sharing the traits of fermentative Gram-negative curved rods with motility and a positive oxidase reaction. Detailed biochemical and genetic characterisation revealed a close affiliation to known species of the marine environment. The first isolate, LP1, is a form of the ubiquitous seawater organism Vibrio splendidus, while the second isolate, LP2, is closely related to V. tapetis (previously only known as the brown ring disease agent in clams). Identification of the new wrasse pathogens V. splendidus LP1 and V. tapetis LP2 is facilitated by break points observed in this study.
分离出了两种对球吻鲈（Symphodus melops）致病的弧菌。致弧菌病的菌株LP1是从1998年挪威西海岸一个遭受弧菌病且日死亡率很高的群体中死亡和濒死的球吻鲈肾脏样本中分离出的优势菌。另一种致弧菌病的菌株LP2是次年从捕获的球吻鲈中分离出来的。再感染实验已证实这些菌株会导致球吻鲈患弧菌病。这两种菌株都是典型的弧菌，具有发酵型革兰氏阴性弯曲杆菌的特征，能运动且氧化酶反应呈阳性。详细的生化和基因特征分析表明它们与海洋环境中的已知物种密切相关。第一个分离株LP1是无处不在的海水生物灿烂弧菌（Vibrio splendidus）的一种形式，而第二个分离株LP2与塔氏弧菌（V. tapetis，以前仅作为蛤类褐环病病原体为人所知）密切相关。本研究中观察到的断点有助于鉴定新的球吻鲈病原体灿烂弧菌LP1和塔氏弧菌LP2。
