Suppr超能文献

选择性聚集肽在巨型单层囊泡中的侧向扩散。

The lateral diffusion of selectively aggregated peptides in giant unilamellar vesicles.

作者信息

Lee Clarence C, Revington Matthew, Dunn Stanley D, Petersen Nils O

机构信息

Department of Chemistry, The University of Western Ontario, London, Canada.

出版信息

Biophys J. 2003 Mar;84(3):1756-64. doi: 10.1016/s0006-3495(03)74983-2.

Abstract

We have systematically investigated the effect of aggregation of a transmembrane peptide on its diffusion in dimyristoylphosphatidylcholine and in palmitoyloleoylphosphatidylcholine model membranes. The hydrophobic segment of the b subunit from E. coli F(1)F(0)-ATP synthase was modified with a histidine tag at the carbonyl terminus and was aggregated selectively by using a series of multivalent, dendritic chelating agents with nitrilotriacetic acid functional groups. Peptide complexes ranging from monomers to hexamers were formed and studied in giant unilamellar vesicles. The rate of diffusion for the transmembrane peptide complexes were found to depend on the size of the complex. The results agree with predictions from the free area model for monomers and dimers, and the hydrodynamic continuum model for tetramers, pentamers, and hexamers. Comparisons with diffusion of lipids confirm that the diffusion of a transmembrane peptide is enhanced by coupling of density fluctuations between the two monolayers.

摘要

我们系统地研究了一种跨膜肽聚集对其在二肉豆蔻酰磷脂酰胆碱和棕榈酰油酰磷脂酰胆碱模型膜中扩散的影响。来自大肠杆菌F(1)F(0)-ATP合酶的b亚基的疏水片段在羰基末端用组氨酸标签进行了修饰,并通过使用一系列带有次氮基三乙酸官能团的多价树枝状螯合剂进行选择性聚集。在巨型单层囊泡中形成并研究了从单体到六聚体的肽复合物。发现跨膜肽复合物的扩散速率取决于复合物的大小。结果与单体和二聚体的自由面积模型以及四聚体、五聚体和六聚体的流体动力学连续介质模型的预测一致。与脂质扩散的比较证实,跨膜肽的扩散通过两个单层之间密度波动的耦合而增强。

相似文献

1
The lateral diffusion of selectively aggregated peptides in giant unilamellar vesicles.
Biophys J. 2003 Mar;84(3):1756-64. doi: 10.1016/s0006-3495(03)74983-2.
2
Nonequilibrium fluctuations of lipid membranes by the rotating motor protein FF-ATP synthase.
Proc Natl Acad Sci U S A. 2017 Oct 24;114(43):11291-11296. doi: 10.1073/pnas.1701207114. Epub 2017 Oct 9.
3
Lateral diffusion of membrane proteins.
J Am Chem Soc. 2009 Sep 9;131(35):12650-6. doi: 10.1021/ja902853g.
4
Direct interaction of subunits a and b of the F0 complex of Escherichia coli ATP synthase by forming an ab2 subcomplex.
J Biol Chem. 2003 Jul 18;278(29):27068-71. doi: 10.1074/jbc.M302027200. Epub 2003 Apr 30.
6
Effect of lipid composition and amino acid sequence upon transmembrane peptide-accelerated lipid transleaflet diffusion (flip-flop).
Biochim Biophys Acta. 2016 Aug;1858(8):1812-20. doi: 10.1016/j.bbamem.2016.04.011. Epub 2016 Apr 27.
7
Genetic complementation between mutant b subunits in F1F0 ATP synthase.
J Biol Chem. 2004 Jul 23;279(30):31205-11. doi: 10.1074/jbc.M404420200. Epub 2004 May 24.
10
Stoichiometry and localization of the stator subunits E and G in Thermus thermophilus H+-ATPase/synthase.
J Biol Chem. 2008 Feb 1;283(5):2595-603. doi: 10.1074/jbc.M704941200. Epub 2007 Nov 30.

引用本文的文献

1
Temperature dependence of membrane viscosity of ternary lipid GUV with L domains.
Biophys J. 2025 Mar 4;124(5):818-828. doi: 10.1016/j.bpj.2025.01.024. Epub 2025 Feb 3.
2
Effects of grafted polymers on the lipid membrane fluidity.
Biophys J. 2024 Feb 20;123(4):489-501. doi: 10.1016/j.bpj.2024.01.014. Epub 2024 Jan 18.
3
Correlated diffusion in lipid bilayers.
Proc Natl Acad Sci U S A. 2021 Nov 30;118(48). doi: 10.1073/pnas.2113202118.
4
Viscosity Landscape of Phase-Separated Lipid Membrane Estimated from Fluid Velocity Field.
Biophys J. 2020 Apr 7;118(7):1576-1587. doi: 10.1016/j.bpj.2020.01.009. Epub 2020 Jan 18.
7
Wanted: scalable tracers for diffusion measurements.
J Phys Chem B. 2014 Nov 13;118(45):12805-17. doi: 10.1021/jp5059885. Epub 2014 Nov 3.
8
Lateral membrane diffusion modulated by a minimal actin cortex.
Biophys J. 2013 Apr 2;104(7):1465-75. doi: 10.1016/j.bpj.2013.02.042.
9
Inferring diffusion in single live cells at the single-molecule level.
Philos Trans R Soc Lond B Biol Sci. 2012 Dec 24;368(1611):20120029. doi: 10.1098/rstb.2012.0029. Print 2013 Feb 5.
10
Dynamic structure formation of peripheral membrane proteins.
PLoS Comput Biol. 2011 Jun;7(6):e1002067. doi: 10.1371/journal.pcbi.1002067. Epub 2011 Jun 23.

本文引用的文献

1
Periodic structures in lipid monolayer phase transitions.
Proc Natl Acad Sci U S A. 1984 May;81(10):3249-53. doi: 10.1073/pnas.81.10.3249.
3
A rapid method of total lipid extraction and purification.
Can J Biochem Physiol. 1959 Aug;37(8):911-7. doi: 10.1139/o59-099.
4
Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells.
Science. 2002 May 3;296(5569):913-6. doi: 10.1126/science.1068539.
6
Lipid rafts and B-cell activation.
Nat Rev Immunol. 2002 Feb;2(2):96-105. doi: 10.1038/nri726.
7
The red-edge effects: 30 years of exploration.
Luminescence. 2002 Jan-Feb;17(1):19-42. doi: 10.1002/bio.671.
8
Roles of lipid rafts in membrane transport.
Curr Opin Cell Biol. 2001 Aug;13(4):470-7. doi: 10.1016/s0955-0674(00)00238-6.
10
Lipid rafts and signal transduction.
Nat Rev Mol Cell Biol. 2000 Oct;1(1):31-9. doi: 10.1038/35036052.

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验