Broom B C, De la Concha E G, Webster A D, Janossy G J, Asherson G L
Clin Exp Immunol. 1976 Jan;23(1):73-7.
The ability of peripheral blood lymphocytes from twenty-two patients with late onset (acquired or common variable) hypogammaglobulinaemia to produce immunoglobulin was assessed by the immunofluorescent detection of intracytoplasmic immunoglobulin (Ic-Ig) in cultures stimulated with pokeweed mitogen (PWM). Intracellular immunoglobulin was found in 4-9-26% of cultured cells from eighteen out of nineteen controls. In contrast nineteen out of twenty-two patients with hypogammaglobulinaemia showed values less than 1% and in ten no Ic-Ig was detected. Two of the remaining three patients showed normal values. Lymphocytes from eleven patients showing less than 1% positive cells were selected for mixture experiments. Lymphocytes from five of the eleven patients strongly depressed immunoglobulin synthesis by normal lymphocytes when mixed together in the presence of PWM. However, lymphocytes from these individual patients did not depress immunoglobulin production in all normal controls.
通过免疫荧光检测在用商陆有丝分裂原（PWM）刺激的培养物中的细胞质内免疫球蛋白（Ic-Ig），评估了22例迟发性（获得性或常见变异型）低丙种球蛋白血症患者外周血淋巴细胞产生免疫球蛋白的能力。在19名对照者中，有18名的培养细胞中有4%-9%-26%发现了细胞内免疫球蛋白。相比之下，22例低丙种球蛋白血症患者中有19例的值低于1%，10例未检测到Ic-Ig。其余3例患者中有2例显示正常数值。选择11例阳性细胞少于1%的患者的淋巴细胞进行混合实验。当在PWM存在的情况下混合在一起时，11例患者中有5例患者的淋巴细胞强烈抑制正常淋巴细胞的免疫球蛋白合成。然而，这些个体患者的淋巴细胞并非在所有正常对照中都抑制免疫球蛋白的产生。
