Serrano María del Carmen, Valverde-Conde Anastasio, Chávez M Mónica, Bernal Samuel, Claro Rosa María, Pemán Javier, Ramirez Mercedes, Martín-Mazuelos Estrella
Servicio de Microbiología Clínica, Hospital Universitario de Valme, Sevilla, Spain.
Diagn Microbiol Infect Dis. 2003 Feb;45(2):131-5. doi: 10.1016/s0732-8893(02)00507-2.
Voriconazole, anidulafungin (VER002, LY303366) and caspofungin are promising antifungal agents which provide a good protection against a variety of fungi, including yeasts and filamentous fungi. In this study, we tested the in vitro efficacy of voriconazole, itraconazole, caspofungin, anidulafungin (VER002, LY303366) and amphotericin B, against different species of Aspergillus spp. isolated from clinical specimens, using a microdilution broth method and following the NCCLS guidelines (document M38-P). We also evaluated the effect that time readings have on MIC results. For caspofungin, we determined the minimun effective concentration (MEC), defined like the lowest concentration of caspofungin causing abnormal hyphal growth. Anidulafungin (VER002, LY303366) was the most active antifungal agent tested with MIC(90) of < or =0,03 mg/L. The activity of voriconazole, and itraconazole very similar with MIC(90) of 0,12 mg/L, 0,12 mg/L respectively. For caspofungin the MEC(90) was of 0,25 mg/L. Amphotericin B was the lest active antifungal agent studied with MIC(90) of 1 mg/L. There were no differences between MIC values at 48 and 72 h. These data demonstrate promising activity of voriconazole, anidulafungin (VER002, LY303366) and caspofungin against Apergillus spp.
伏立康唑、阿尼芬净（VER002，LY303366）和卡泊芬净都是很有前景的抗真菌药物，它们能有效抵御多种真菌，包括酵母菌和丝状真菌。在本研究中，我们采用微量稀释肉汤法并遵循美国国家临床实验室标准委员会（NCCLS）指南（文件M38 - P），测试了伏立康唑、伊曲康唑、卡泊芬净、阿尼芬净（VER002，LY303366）和两性霉素B对从临床标本中分离出的不同曲霉菌种的体外抗菌效果。我们还评估了不同时间读数对最低抑菌浓度（MIC）结果的影响。对于卡泊芬净，我们测定了最低有效浓度（MEC），其定义为导致菌丝生长异常的卡泊芬净最低浓度。阿尼芬净（VER002，LY303366）是所测试的最有效的抗真菌药物，其MIC（90）≤0.03mg/L。伏立康唑和伊曲康唑的活性非常相似，MIC（90）分别为0.12mg/L和0.12mg/L。卡泊芬净的MEC（90）为0.25mg/L。两性霉素B是所研究的活性最低的抗真菌药物，MIC（90）为1mg/L。48小时和72小时的MIC值之间没有差异。这些数据表明伏立康唑、阿尼芬净（VER002，LY303366）和卡泊芬净对曲霉菌种具有良好的抗菌活性。
