Camejo M I
Universidad Simón Bolívar, Departamento de Biología de Organismos, Caracas, Venezuela.
Arch Androl. 2003 Mar-Apr;49(2):111-6. doi: 10.1080/01485010390129232.
In vitro studies have reported that seminal plasma of fertile men has more inhibitory mitogenic effect and higher suppressive activity on antibody-dependent cellular cytotoxicity than seminal plasma from infertile males. Is it possible that variations in the seminal plasma composition could explain these results? The present study was conducted to investigate the relation between immunosuppressive PGE(2) and IL-10 to pro-inflammatory IL-6 in seminal plasma of infertile and fertile men. Utilizing specific ELISA commercial kit, semen samples from 45 infertile undiagnosed and 25 fertile men were examined for the presence of PGE(2), IL-10, and IL-6. The IL-6 concentration in seminal plasma of infertile men was significantly higher than that of fertile men (p<.0 4). However, levels of IL-10 were lower for the semen of infertile men than that of fertile men (p<.04). No differences were observed in PGE(2) concentrations between both groups. Increase in pro-inflammatory cytokines, such as IL-6, and decrease in immunosuppressive ones, such as IL-10, could alter the tolerance to sperm cell in male and female tracts and reduce the favorable conditions to reach the fecundation and implantation. It is necessary to conduct further studies that include other pro- and anti-inflammatory substances to evaluate the importance of the immune balance of semen in human fertility.
体外研究报告称，与不育男性的精浆相比，可育男性的精浆对抗体依赖性细胞毒性具有更强的抑制促有丝分裂作用和更高的抑制活性。精浆成分的差异能否解释这些结果呢？本研究旨在调查不育和可育男性精浆中免疫抑制性前列腺素E2（PGE2）和白细胞介素10（IL-10）与促炎性白细胞介素6（IL-6）之间的关系。使用特定的酶联免疫吸附测定（ELISA）商业试剂盒，对45名未确诊的不育男性和25名可育男性的精液样本进行检测，以确定PGE2、IL-10和IL-6的存在情况。不育男性精浆中的IL-6浓度显著高于可育男性（p<0.04）。然而，不育男性精液中的IL-10水平低于可育男性（p<0.04）。两组之间的PGE2浓度未观察到差异。促炎性细胞因子（如IL-6）增加，而免疫抑制性细胞因子（如IL-10）减少，可能会改变男性和女性生殖道对精子细胞的耐受性，并减少受孕和着床的有利条件。有必要开展进一步研究，纳入其他促炎和抗炎物质，以评估精液免疫平衡在人类生育中的重要性。
