Sisay T, Zerihun A
Department of Microbiology, Infectious Diseases and Veterinary Public Health, Faculty of Veterinary Medicine, Addis Ababa University, Debre Zeit, Ethiopia.
Vet Res Commun. 2003 Jan;27(1):3-14. doi: 10.1023/a:1022088005887.
The prevalence and serotypic diversity of Mannheimia [Pasteurella] haemolytica and Pasteurella trehalosi from nasal swabs, sera and abattoir specimens from sheep in the highlands of Wollo, North East Ethiopia was investigated. Prevalence rates of 83% and 75% of these microorganisms were found in the serum samples and nasal swabs, respectively, from apparently healthy sheep. In a local abattoir, 205 lungs were investigated, 34% of which showed pneumonia, from which samples were collected from 51 lungs and the same number of corresponding tonsils. Mannheimia and Pasteurella species were isolated from 59% of these pneumonic lungs and 69% of the respective tonsils. M. haemolytica serotypes accounted for 41 (59%) and P. trehalosi for 11 (32%) of the isolates from the abattoir specimens. The majority (67%) of isolates from nasal swabs were P. trehalosi, M. haemolytica being isolated f rom 4 (13%) of the swabs. M. glucosida was isolated only from the tonsils. The predominant serotypes of the isolates from both the nasal swabs and the abattoir specimens were M. haemolytica A1 (17%) and P. trehalosi T4 (16%) and T3 (13%). P. trehalosi T15 was less commonly encountered, while M. haemolytica A9 and A13 were not isolated. Studies on sera from 100 sheep indicated that antibodies against M. haemolytica serotype A1 (14%) were most common, followed by A5 and A8 (each 10%) and A9 and P. trehalosi T3 (each 9%) and T4 (8%). Antibodies against M. glucosida or serotype All occurred in 2% of the sera. Multiple serotypes were common in all types of samples. The importance of including in vaccines the most prevalent serotypes involved in the pneumonia of sheep in the area is discussed.
对埃塞俄比亚东北部沃洛高地绵羊的鼻拭子、血清和屠宰场样本中的溶血曼氏杆菌（以前称为溶血巴氏杆菌）和海藻糖巴氏杆菌的流行情况及血清型多样性进行了调查。在看似健康的绵羊血清样本和鼻拭子中，分别发现这些微生物的流行率为83%和75%。在当地一家屠宰场，对205个肺脏进行了调查，其中34%表现出肺炎，从51个肺脏及其相应数量的扁桃体中采集了样本。从这些肺炎肺脏的59%和相应扁桃体的69%中分离出了曼氏杆菌和巴氏杆菌属。来自屠宰场样本的分离株中，溶血曼氏杆菌血清型占41株（59%），海藻糖巴氏杆菌占11株（32%）。鼻拭子分离株的大多数（67%）是海藻糖巴氏杆菌，4份（13%）鼻拭子中分离出了溶血曼氏杆菌。仅从扁桃体中分离出了葡萄糖苷曼氏杆菌。鼻拭子和屠宰场样本分离株的主要血清型是溶血曼氏杆菌A1（17%）、海藻糖巴氏杆菌T4（16%）和T3（13%）。海藻糖巴氏杆菌T15较少见，而未分离出溶血曼氏杆菌A9和A13。对100只绵羊血清的研究表明，抗溶血曼氏杆菌血清型A1的抗体（14%）最为常见，其次是A5和A8（各10%）以及A9和海藻糖巴氏杆菌T3（各9%）和T4（8%）。抗葡萄糖苷曼氏杆菌或血清型A11的抗体出现在2%的血清中。多种血清型在所有类型的样本中都很常见。文中讨论了在疫苗中纳入该地区绵羊肺炎中最普遍的血清型的重要性。
