Tesfaye Biruk, Sisay Tessema Tesfaye, Tefera Genene
Department of Microbiology, Immunology and Veterinary Public Health, School of Veterinary Medicine, Addis Ababa University, Debre Zeit, Ethiopia.
Trop Anim Health Prod. 2013 Jun;45(5):1243-9. doi: 10.1007/s11250-013-0355-x. Epub 2013 Jan 19.
A study was conducted to isolate bacterial species/pathogens from the nasal cavity of apparently healthy and pneumonic sheep. Nasal swabs were collected aseptically, transported in tryptose soya broth and incubated for 24 h. Then, each swab was streaked onto chocolate and blood agar for culture. Bacterial species were identified following standard bacteriological procedures. Accordingly, a total of 1,556 bacteria were isolated from 960 nasal swabs collected from three different highland areas of Ethiopia, namely Debre Berhan, Asella, and Gimba. In Debre Berhan, 140 Mannheimia haemolytica, 81 Histophilus somni, 57 Staphylococcus species, and 52 Bibersteinia trehalosi were isolated. While from Gimba M. haemolytica, Staphylococcus, Streptococcus, and H. somni were isolated at rates of 25.2, 15.9, 11.4, and 5.9 %, respectively, of the total 647 bacterial species. In Asella from 352 bacterial species isolated, 93 (26.4 %) were M. haemolytica, 48 (13.6 %) were Staphylococcus species, 26 (7.4 %) were B. trehalosi, and 17 (4.8 %) H. somni were recognized. Further identification and characterization using BIOLOG identification system Enterococcus avium and Sphingomonas sanguinis were identified at 100 % probability, while, H. somni and Actinobacillus lignerisii were suggested by the system. The study showed that a variety of bacterial species colonize the nasal cavity of the Ethiopian highland sheep with variable proportion between healthy and pneumonic ones. To our knowledge, this is the first report on isolation of H. somni, an important pathogen in cattle, from the respiratory tract of a ruminant species in the country.
开展了一项研究，旨在从看似健康和患肺炎的绵羊鼻腔中分离细菌种类/病原体。无菌采集鼻拭子，置于胰蛋白胨大豆肉汤中运送，并培养24小时。然后，将每个拭子划线接种于巧克力琼脂和血琼脂上进行培养。按照标准细菌学程序鉴定细菌种类。据此，从埃塞俄比亚三个不同高原地区（即德布雷伯汉、阿塞拉和金巴）采集的960份鼻拭子中，共分离出1556株细菌。在德布雷伯汉，分离出140株溶血曼氏杆菌、81株睡眠嗜组织菌、57株葡萄球菌属细菌和52株苏云金毕赤酵母。而在金巴，从总共647株细菌中分别分离出溶血曼氏杆菌、葡萄球菌、链球菌和睡眠嗜组织菌，分离率分别为25.2%、15.9%、11.4%和5.9%。在阿塞拉，从分离出的352株细菌中，93株（26.4%）为溶血曼氏杆菌，48株（13.6%）为葡萄球菌属细菌，26株（7.4%）为苏云金毕赤酵母，17株（4.8%）为睡眠嗜组织菌。使用BIOLOG鉴定系统进一步鉴定和表征，以100%的概率鉴定出鸟肠球菌和血鞘氨醇单胞菌，而该系统提示存在睡眠嗜组织菌和木质放线杆菌。该研究表明，埃塞俄比亚高原绵羊鼻腔中定殖有多种细菌种类，健康绵羊和患肺炎绵羊中的比例各不相同。据我们所知，这是该国首次从反刍动物呼吸道中分离出牛的一种重要病原体——睡眠嗜组织菌的报告。
