Tadjoedin E S, de Lange G L, Bronckers A L J J, Lyaruu D M, Burger E H
Department of Oral Cell Biology, Academic Center for Dentistry Amsterdam, Amsterdam, The Netherlands.
J Clin Periodontol. 2003 Mar;30(3):261-70. doi: 10.1034/j.1600-051x.2003.01099.x.
To study in detail the performance of deproteinized cancellous bovine bone (DPBB, Bio-Osso) granules as a bone substitute, a histomorphometric was performed on five patients treated with DPBB for reconstruction of the severely atrophic maxilla.
DPBB was used as mixture with autogenous bone particles, in concentrations that increased from 20% to 100% DPBB, with the time of healing increasing accordingly from 5 to 8 months. A total of 20 vertical biopsies was taken at the time of fixture installation and used for histomorphometry as undecalcified Goldner stained sections.
The results show that in all cases, the DPBB granules had been interconnected by bridges of vital newly formed bone. The volume of bone in the grafted area correlated inversely with the concentration of DPBB grafted, and varied between 37% and 23%. However, the total volume of mineralized material (bone plus DPI3B granules) remained within the same range in all five patients (between 53% and 59%). The high values for osteoid and resorption surface, and the presence of tartrate-resistant acid phosphatase-positive multinucleated osteoclasts in resorption lacunae, indicated that bone remodeling was very active in all grafts. Osteoclasts were also observed in shallow resorption pits on DPBB surfaces. The percentage DPBB surface in contact with bone remained stable at about 35% and could not be related to the proportion of DPBB grafted.
Although the number of patients examined was limited, the data suggest that deproteinized cancellous bovine bone, preferably combined with autogenous bone particles, is a suitable material for sinus floor elevation in the severely atrophic human maxilla.
为详细研究脱蛋白松质牛骨（DPBB，Bio - Osso）颗粒作为骨替代物的性能，对5例接受DPBB治疗严重萎缩性上颌骨重建的患者进行了组织形态计量学研究。
DPBB与自体骨颗粒混合使用，浓度从20%增至100% DPBB，愈合时间相应从5个月增至8个月。在种植体植入时共采集20份垂直活检样本，用于组织形态计量学分析，制成未脱钙的戈德纳染色切片。
结果表明，在所有病例中，DPBB颗粒已通过新生活性骨桥相互连接。移植区域的骨体积与移植的DPBB浓度呈负相关，在37%至23%之间变化。然而，所有5例患者矿化物质（骨加DPBB颗粒）的总体积保持在相同范围内（53%至59%之间）。类骨质和吸收表面的高值，以及吸收陷窝中抗酒石酸酸性磷酸酶阳性多核破骨细胞的存在，表明所有移植骨中骨重塑非常活跃。在DPBB表面的浅吸收坑中也观察到破骨细胞。与骨接触的DPBB表面百分比保持在约35%稳定，且与移植的DPBB比例无关。
尽管检查的患者数量有限，但数据表明脱蛋白松质牛骨，最好与自体骨颗粒联合使用，是严重萎缩性人类上颌骨窦底提升的合适材料。
