Eschen-Lippold Lennart, Rothe Grit, Stumpe Michael, Göbel Cornelia, Feussner Ivo, Rosahl Sabine
Department of Stress and Developmental Biology, Leibniz Institute of Plant Biochemistry, Weinberg 3, D-06120 Halle/Saale, Germany.
Phytochemistry. 2007 Mar;68(6):797-801. doi: 10.1016/j.phytochem.2006.12.010. Epub 2007 Jan 26.
Oxygenated polyunsaturated fatty acids synthesized via the lipoxygenase pathway play a role in plant responses to pathogen attack. In solanaceous plants, the preferential stimulation of the 9-lipoxygenase pathway in response to pathogen infection leads to the formation of the divinyl ether-containing polyunsaturated fatty acids colneleic and colnelenic acid, as well as hydroxy and trihydroxy polyunsaturated fatty acids. To functionally assess the role of divinyl ethers, transgenic potato plants were generated which express an RNA interference construct directed against the pathogen-inducible 9-divinyl ether synthase. Efficient reduction of 9-divinyl ether synthase transcript accumulation correlated with reduced levels of colneleic and colnelenic acid. However, in response to infection with virulent Phytophthora infestans, the causal agent of late blight disease, no significant differences in pathogen biomass could be detected suggesting that the levels of antimicrobial divinyl ethers are not critical for defense against Phytophthora infestans in a compatible interaction.
通过脂氧合酶途径合成的氧化多不饱和脂肪酸在植物对病原体攻击的反应中发挥作用。在茄科植物中，响应病原体感染时9-脂氧合酶途径的优先激活会导致含二乙烯基醚的多不饱和脂肪酸科奈酸和科奈连酸以及羟基和三羟基多不饱和脂肪酸的形成。为了从功能上评估二乙烯基醚的作用，构建了表达针对病原体诱导型9-二乙烯基醚合酶的RNA干扰构建体的转基因马铃薯植株。9-二乙烯基醚合酶转录物积累的有效减少与科奈酸和科奈连酸水平的降低相关。然而，在感染致病疫霉（晚疫病的病原体）后，未检测到病原体生物量有显著差异，这表明在亲和互作中，抗菌二乙烯基醚的水平对于抵御致病疫霉并不关键。
