Mulayim Naciye, Palter Steven F, Selam Belgin, Arici Aydin
Department of Obstetrics and Gynecology, Yale University School of Medicine, New Haven, Conn 06520, USA.
Am J Obstet Gynecol. 2003 Mar;188(3):651-6. doi: 10.1067/mob.2003.135.
The human fallopian tube creates the microenvironment for fertilization and early embryogenesis. Salpingitis may result in infertility and ectopic pregnancy by causing tubal blockage and hydrosalpinx. To better understand the relationship between infectious inflammation and tubal damage, we investigated the expression and regulation of interleukin-8 in human tubal epithelial and stromal cells in culture.
Human fallopian tube epithelial and stromal cell cultures were used to measure interleukin-8 messenger RNA and interleukin-8 protein levels at basal conditions and after stimulation with interleukin-1alpha and tumor necrosis factor-alpha. Northern blot analysis and enzyme-linked immunosorbent assay were used to evaluate messenger RNA and protein levels, respectively.
Tubal epithelial cells expressed high levels of interleukin-8 messenger RNA and secreted significantly more immunoreactive interleukin-8 into culture medium than did tubal stromal cells (2065 +/- 153 pg/mg vs 530 +/- 56 pg/mg of total protein, P <.01). Interleukin-1alpha and TNF-alpha treatments induced a concentration-dependent increase in interleukin-8 messenger RNA expression in both epithelial and stromal cells. However, at the protein level, although interleukin-1alpha and tumor necrosis factor-alpha treatments increased the secretion of interleukin-8 from stromal cells significantly, similar treatments had no effect on interleukin-8 secretion from epithelial cells.
The expression of interleukin-8 in human tubal epithelial and stromal cells is different. Interleukin-8 expression of tubal epithelial and stromal cells in response to inflammatory cytokines such as interleukin-1alpha and tumor necrosis factor-alpha also varies. This may be important in the pathogenesis of salpingitis.
人类输卵管为受精和早期胚胎发育创造微环境。输卵管炎可通过导致输卵管阻塞和输卵管积水而导致不孕和异位妊娠。为了更好地理解感染性炎症与输卵管损伤之间的关系，我们研究了培养的人输卵管上皮细胞和基质细胞中白细胞介素-8的表达和调控。
用人输卵管上皮细胞和基质细胞培养物来测量基础条件下以及用白细胞介素-1α和肿瘤坏死因子-α刺激后白细胞介素-8信使核糖核酸和白细胞介素-8蛋白水平。分别用Northern印迹分析和酶联免疫吸附测定来评估信使核糖核酸和蛋白水平。
输卵管上皮细胞表达高水平的白细胞介素-8信使核糖核酸，并且向培养基中分泌的免疫反应性白细胞介素-8明显多于输卵管基质细胞（每毫克总蛋白中为2065±153皮克对530±56皮克，P<.01）。白细胞介素-1α和肿瘤坏死因子-α处理诱导上皮细胞和基质细胞中白细胞介素-8信使核糖核酸表达呈浓度依赖性增加。然而，在蛋白水平上，尽管白细胞介素-1α和肿瘤坏死因子-α处理显著增加了基质细胞中白细胞介素-8的分泌，但类似处理对上皮细胞中白细胞介素-8的分泌没有影响。
人输卵管上皮细胞和基质细胞中白细胞介素-8的表达不同。输卵管上皮细胞和基质细胞对白细胞介素-1α和肿瘤坏死因子-α等炎性细胞因子的白细胞介素-8表达也有所不同。这可能在输卵管炎的发病机制中具有重要意义。
