Zahiruddin Sultan, Parveen Abida, Khan Washim, Parveen Rabea, Ahmad Sayeed
Bioactive Natural Product Laboratory, Department of Pharmacognosy and Phytochemistry, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi 110062, India.
Department of Clinical Research, School of Interdisciplinary Sciences and Technology, Jamia Hamdard, New Delhi 110062, India.
Evid Based Complement Alternat Med. 2021 Dec 31;2021:2847440. doi: 10.1155/2021/2847440. eCollection 2021.
We aimed to develop a chromatographic method for scientific validation of water extract of some important Indian traditional plants used in AYUSH-based formulation as immunomodulator and to evaluate their bioactive potential. Fruits of L. and L., stem of (Willd.) Miers, rhizome of L., leaves of L. and L., roots of L., and stem bark of A. Juss. were coarsely powdered and extracted in three different solvents (water, ethanol, and hydroethanol). The antioxidant potential was determined through 1, 1-diphenyl-2-picrylhydrazyl and ferric reducing capacity methods. Thin-layer chromatography (TLC) was carried out for the comparative metabolite profiling of the extracts using toluene, ethyl acetate, and formic acid (5 : 4 : 1, v/v/v) as a solvent system. immunomodulatory activity of the extracts has been tested on splenocyte proliferation and pinocytic assay. Hydroethanolic extract (HEE) of most of the plant materials has the highest phenolic and flavonoid contents, followed by water extract (WE) and ethanolic extract (EE), whereas the water extracts of most of the plant material showed better antioxidant activity. Almost all extract exhibited splenocyte proliferation and pinocytic activity in a dose-dependent manner. But water extract showed significantly higher splenocyte proliferation and pinocytic activity as compared to the other two extracts. TLC analysis resulted in detection of totally 63 and 56 metabolites at 254 nm and 366 nm, respectively. Through principal component analysis (PCA), it was observed that metabolite pattern of different extracts from same plant materials may be different or similar. This preliminary result can be used for quality evaluation and to develop a synergy-based polyherbal combination of water extracts of selected plant materials.
我们旨在开发一种色谱方法，用于科学验证一些用于阿育吠陀配方的重要印度传统植物的水提取物作为免疫调节剂，并评估其生物活性潜力。将某植物和某植物的果实、某植物（威尔德）米尔斯的茎、某植物的根茎、某植物和某植物的叶子、某植物的根以及某植物朱斯的茎皮粗粉，并在三种不同溶剂（水、乙醇和氢乙醇）中提取。通过1,1-二苯基-2-苦基肼和铁还原能力方法测定抗氧化潜力。使用甲苯、乙酸乙酯和甲酸（5 : 4 : 1，v/v/v）作为溶剂系统，对提取物进行薄层色谱（TLC）分析以进行比较代谢物谱分析。已对提取物的免疫调节活性进行了脾细胞增殖和吞噬试验测试。大多数植物材料的氢乙醇提取物（HEE）具有最高的酚类和黄酮类含量，其次是水提取物（WE）和乙醇提取物（EE），而大多数植物材料的水提取物显示出更好的抗氧化活性。几乎所有提取物均以剂量依赖性方式表现出脾细胞增殖和吞噬活性。但与其他两种提取物相比，水提取物显示出明显更高的脾细胞增殖和吞噬活性。TLC分析分别在254 nm和366 nm处检测到总共63种和56种代谢物。通过主成分分析（PCA）观察到，来自相同植物材料的不同提取物的代谢物模式可能不同或相似。这一初步结果可用于质量评估，并开发基于协同作用的所选植物材料水提取物的多草药组合。
