Zhang Xiao Gang, Chen Yun Zhen, Lei Han, Wang Zhou Bi
Department of Cardiology, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China.
Zhonghua Gan Zang Bing Za Zhi. 2003 Feb;11(2):77-9.
To investigate the relationship between hepatic lipase activity and fatty degeneration of hepatocytes and explore the effects of tea polyphennols (TP) on the changes of hepatic lipase (HL) activity in rabbits with fatty liver.
According to serum cholesterol and triglyceride (TC) levels, 19 rabbits were divided into fatty liver group (FL, n=6) fed with high cholesterol diet, TP group (n=7) fed with high cholesterol diet and 20mug/g/d tea polyphennols everyday orally, control group (n=6) fed with normal diet. After 8 weeks, the levels of serum TC, HL activity, HL activity and malondildehyde (MDA) in hepatic tissue were detected, and the pathomorphology of hepatic tissue were determined in all rabbits.
The fatty degeneration of hepatocyts in FL group was more severe than that in TP and control group. The serum TC level in TP group (16.87 6.58) mmol/L was higher than that (1.11 0.82) mmol/L in control group (t=5.786, p<0.05), but lower than that (28.49 5.99) mmol/L in FL group (t=3.968, p<0.05). The serum low density lipoprotein-cholesterol level in Tp group (5.10 4.19) mmol/L also higher than that (0.71 1.14) mmol/L in control group (t=3.763, p<0.05), but lower than that (12.15 1.95) mmol/L in FL group (t=2.478, p<0.05). The number of positive dots presenting HL activity level in 100 square micron, hepatic tissue in TP group (3.24 0.17) was higher than that (1.76 0.10) in FL group (t=-3.153, p<0.05), but lower than that (4.14 0.05) in control group (t=-2.902, p<0.05). The levels of MDA in hepatic tissue in TP group (44.66 26.18) nmol/mg was significantly lower than that (75.58 29.88) nmol/mg in FL group (t=2.261, p<0.05), but no evidently different from that (43.64 16.95) nmol/mg in control group. The plasma HL activity was no difference among the three groups.
The HL activity in hepatic tissue with fatty degeneration of hepatocytes was lower than that in normal liver. Tea polyphennols can increase HL activity in hepatic tissue and protect hepatocytes from fatty degeneration.
探讨肝脂肪酶活性与肝细胞脂肪变性之间的关系，并探究茶多酚（TP）对脂肪肝家兔肝脂肪酶（HL）活性变化的影响。
根据血清胆固醇和甘油三酯（TC）水平，将19只家兔分为高脂饮食喂养的脂肪肝组（FL，n = 6）、高脂饮食且每日口服20μg/g/d茶多酚的TP组（n = 7）、正常饮食喂养的对照组（n = 6）。8周后，检测所有家兔的血清TC水平、HL活性、肝组织中的HL活性和丙二醛（MDA）水平，并确定肝组织的病理形态学。
FL组肝细胞的脂肪变性比TP组和对照组更严重。TP组血清TC水平（16.87±6.58）mmol/L高于对照组（1.11±0.82）mmol/L（t = 5.786，p < 0.05），但低于FL组（28.49±5.99）mmol/L（t = 3.968，p < 0.05）。TP组血清低密度脂蛋白胆固醇水平（5.10±4.19）mmol/L也高于对照组（0.71±1.14）mmol/L（t = 3.763，p < 0.05），但低于FL组（12.15±1.95）mmol/L（t = 2.478，p < 0.05）。TP组肝组织中每100平方微米呈现HL活性水平的阳性点数（3.24±0.17）高于FL组（1.76±0.10）（t = -3.153，p < 0.05），但低于对照组（4.14±0.05）（t = -2.902，p < 0.05）。TP组肝组织中MDA水平（44.66±26.18）nmol/mg显著低于FL组（75.58±29.88）nmol/mg（t = 2.261，p < 0.05），但与对照组（43.64±16.95）nmol/mg无明显差异。三组血浆HL活性无差异。
肝细胞脂肪变性的肝组织中HL活性低于正常肝脏。茶多酚可增加肝组织中HL活性并保护肝细胞免受脂肪变性。
