Tang Yuan-ting, Guan Xiao-qin, Liu Li, Liu Lin, Wang Li-juan
Department of Pathology, College of Basic Medicine, Chongqing Medical University, Chongqing, 400016, China.
Zhonghua Gan Zang Bing Za Zhi. 2009 Apr;17(4):301-5.
To investigate the effect of tea polyphenols (TP) on expression of nuclear factor kappa B (NF-kB) in rats with alcoholic liver diseases and in cells treated with alcohol.
22 female Wistar rats were randomly divided into three groups: a control group, an alcohol model group and a TP plus alcohol group. All treatments were injected into stomach through intragastric tube. L02 cells were divided into five groups: a control group, an alcohol treated group, a prevention group (cells were treated with TP for 3 days, and then treated with alcohol), an intervention group (cells treated with TP and alcohol), and a therapeutic group (cells were treated with alcohol for 3 days, and then treated with TP). Histopathology was observed under light microscope (LM); serum MDA, ROS in cells were quantified by optical density measurement; the expression of NF-kB and IkB was determined by RT-PCR; and the activity of NF-kB was checked with Electrophoretic Mobility Shift Assay (EMSA).
LM indicated hepatocytes were injured obviously in the model group. Serum MDA and cells ROS in TP treated groups were significantly lower than the alcohol treated group. The level of NF-kB mRNA expression in TP treated groups(rats: 0.58+/-0.16, cells: 0.60+/-0.03, 0.59+/-0.01, 0.59+/-0.01) were significantly lower than the alcohol treated group (rats: 1.15+/-0.03, cells: 0.76+/-0.03) (P<0.01), the level of IkB mRNA expression in the prevention group, intervention group, and therapeutic group (0.51+/-0.01, 0.50+/-0.01, 0.50+/-0.12) were significantly higher than the alcohol treated group (0.61+/-0.03) (P<0.05), the difference among the three groups was not significant (P>0.05). The activity of NF-kB in TP treated rats(DNA stain: 669.85+/-41.34, Protein stain: 675.35+/-18.27) was significantly lower than the alcohol treated rats(DNA stain: 1410.78+/-22.19, Protein stain:1426.08+/-33.15) (P<0.01); NF-kB activity in cells of the prevention, intervention, therapeutic groups (DNA stain: 713.07+/-11.91, 710.79+/-14.99, 693.45+/-71.69; Protein stain: 758.88+/-34.65, 753.07+/-76.78, 725.77+/-36.09) was significantly lower than the alcohol treated cells (DNA stain: 849.94+/-12.45, Protein stain: 925.96+/-5.78) (P<0.01), the difference among the three TP treated groups was not significant (P>0.01).
TP can alleviate and prevent alcohol-induced liver injury via inhibiting NF-kB activation.
探讨茶多酚（TP）对酒精性肝病大鼠及酒精处理细胞中核因子κB（NF-κB）表达的影响。
将22只雌性Wistar大鼠随机分为三组：对照组、酒精模型组和TP加酒精组。所有处理均通过胃管灌胃给药。将L02细胞分为五组：对照组、酒精处理组、预防组（细胞先用TP处理3天，然后用酒精处理）、干预组（细胞用TP和酒精处理）和治疗组（细胞先用酒精处理3天，然后用TP处理）。在光学显微镜（LM）下观察组织病理学；通过光密度测量定量血清丙二醛（MDA）、细胞中的活性氧（ROS）；通过逆转录聚合酶链反应（RT-PCR）测定NF-κB和IkB的表达；用凝胶迁移率变动分析（EMSA）检测NF-κB的活性。
LM显示模型组肝细胞明显受损。TP处理组的血清MDA和细胞ROS显著低于酒精处理组。TP处理组（大鼠：0.58±0.16，细胞：0.60±0.03、0.59±0.01、0.59±0.01）的NF-κB mRNA表达水平显著低于酒精处理组（大鼠：1.15±0.03，细胞：0.76±0.03）（P<0.01），预防组、干预组和治疗组（0.51±0.01、0.50±0.01、0.50±0.12）的IkB mRNA表达水平显著高于酒精处理组（0.61±0.03）（P<0.05），三组之间差异不显著（P>0.05）。TP处理大鼠中NF-κB的活性（DNA染色：669.85±41.34，蛋白质染色：675.35±18.27）显著低于酒精处理大鼠（DNA染色：1410.78±22.19，蛋白质染色：1426.08±33.15）（P<0.01）；预防组、干预组、治疗组细胞中NF-κB活性（DNA染色：713.07±11.91、710.79±14.99、693.45±71.69；蛋白质染色：758.88±34.65、753.07±76.78、725.77±36.09）显著低于酒精处理细胞（DNA染色：849.94±12.45，蛋白质染色：925.96±5.78）（P<0.01），三个TP处理组之间差异不显著（P>0.01）。
TP可通过抑制NF-κB激活减轻和预防酒精性肝损伤。
