Dutta Hiran M, Arends Dane A
Department of Biological Sciences, Kent State University, Kent, OH 44242, USA.
Environ Res. 2003 Mar;91(3):157-62. doi: 10.1016/s0013-9351(02)00062-2.
The effects of endosulfan upon brain acetylcholinesterase (AChE) activity were measured in juvenile bluegill sunfish (Lepomis macrochirus). Based on exposure durations of 0, 24, 48, 72, and 96h and 1 week at 1.0 microg/L (just below the LC50 of 1.2 microg/L for this species), step-wise decreases in AChE activity were noted, corresponding to 0%, 3.57%, 12.65%, 14.23%, 16.31%, and 23.11% inhibition, respectively. Total brain protein concentrations were measured to test the accuracy of the AChE data with no significant anomalies. The duration of exposure was related to the reduction in the AChE activities which reflected the biotoxicity of endosulfan. The changes in the AChE activities will certainly affect the normal behavior of the juvenile bluegill which is detrimental to their very existence in the natural habitat.
在幼年蓝鳃太阳鱼（Lepomis macrochirus）中测定了硫丹对脑乙酰胆碱酯酶（AChE）活性的影响。基于在1.0微克/升（略低于该物种1.2微克/升的半数致死浓度）下0、24、48、72和96小时以及1周的暴露时间，观察到AChE活性呈逐步下降，分别对应0%、3.57%、12.65%、14.23%、16.31%和23.11%的抑制率。测量了全脑蛋白质浓度以检验AChE数据的准确性，未发现显著异常。暴露时间与AChE活性的降低有关，这反映了硫丹的生物毒性。AChE活性的变化肯定会影响幼年蓝鳃太阳鱼的正常行为，这对它们在自然栖息地的生存是有害的。
