Undergraduate Program in Biological Sciences, Universidade Estadual do Oeste do Paraná, Cascavel, PR, Brazil.
Graduate Program in Conservation and Management of Natural Resources Postgraduate Program, Universidade Estadual do Oeste do Paraná, Cascavel, PR, Brazil.
Ecotoxicology. 2020 Apr;29(3):314-326. doi: 10.1007/s10646-020-02176-6. Epub 2020 Feb 27.
Anthropogenic activities promote changes in community structure and decrease the species abundance of amphibians. The aim of this study was to assess potential alterations in the antioxidant system and cholinesterase activity, histopathological and oxidative damage in Lithobates catesbeianus tadpoles exposed to water from the Cascavel River, in Southern Brazil. Water samples (140 L each) were collected from the headwater, urban and rural areas of the river. Tadpoles were acclimated for seven days. After acclimatization tadpoles were reared in water from the river, except for the control aquarium. After seven days, a portion of the liver was removed and prepared for cholinesterase (ChE), superoxide dismutase (SOD), catalase (CAT) and lipid peroxidation (LPO) analysis; another part of the tissue was prepared for histological examination. An elevation of CAT activity was observed for water from both urban and rural environments. A decrease in LPO reaction was detected, mainly among the tadpoles exposed to water from the rural area. These alternations can cause delay the metamorphosis and lead to metabolic dysfunction, interfering in survival capacity and diminishing, not only individual fitness, but that of the whole population.
人为活动促进了群落结构的变化，降低了两栖动物的物种丰富度。本研究旨在评估南方巴西卡塞雷斯河水源中抗氧化系统和胆碱酯酶活性、组织病理学和氧化损伤的潜在变化对 Lithobates catesbeianus 蝌蚪的影响。从河流的源头、城市和农村地区采集了 140 升水样。蝌蚪经过七天的适应期后，在河水中（除对照组外）进行了为期七天的养殖。七天后，取出一部分肝脏用于检测胆碱酯酶（ChE）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和脂质过氧化（LPO）；另一部分组织用于组织学检查。城市和农村地区的水样均观察到 CAT 活性升高。LPO 反应降低，主要发生在暴露于农村地区水样的蝌蚪中。这些变化可能导致变态延迟和代谢功能障碍，不仅影响个体适应性，还影响整个种群的生存能力。
