Tang Peijun, Li Yuandong, Rakymzhan Adiya, Xie Zhiying, Wang Ruikang K
Department of Bioengineering, University of Washington, 3720 15th Ave NE, Seattle, WA 98195, USA.
Biomed Opt Express. 2020 Jan 9;11(2):699-710. doi: 10.1364/BOE.381332. eCollection 2020 Feb 1.
We describe a method to measure tissue dynamics in mouse barrel cortex during functional activation via phase-sensitive optical coherence tomography (PhS-OCT). The method measures the phase changes in OCT signals, which are induced by the tissue volume change, upon which to localize the activated tissue region. Phase unwrapping, compensation and normalization are applied to increase the dynamic range of the OCT phase detection. To guide the OCT scanning, intrinsic optical signal imaging (IOSI) system equipped with a green light laser source (532 nm) is integrated with the PhS-OCT system to provide a full field time-lapsed images of the reflectance that is used to identify the transversal 2D localized tissue response in the mouse brain. The OCT results show a localized decrease in the OCT phase signal in the activated region of the mouse brain tissue. The decrease in the phase signal may be originated from the brain tissue compression caused by the vasodilatation in the activated region. The activated region revealed in the cross-sectional OCT image is consistent with that identified by the IOSI imaging, indicating the phase change in the OCT signals may associate with the changes in the corresponding hemodynamics. localized tissue dynamics in the barrel cortex at depth during whisker stimulation is observed and monitored in this study.
我们描述了一种通过相敏光学相干断层扫描（PhS-OCT）在功能激活期间测量小鼠桶状皮质组织动力学的方法。该方法测量由组织体积变化引起的OCT信号中的相位变化，据此定位激活的组织区域。应用相位展开、补偿和归一化来增加OCT相位检测的动态范围。为了指导OCT扫描，配备绿色激光源（532 nm）的固有光学信号成像（IOSI）系统与PhS-OCT系统集成，以提供用于识别小鼠大脑中横向二维局部组织反应的反射率全场延时图像。OCT结果显示小鼠脑组织激活区域的OCT相位信号局部降低。相位信号的降低可能源于激活区域血管舒张引起的脑组织压缩。横截面OCT图像中显示的激活区域与IOSI成像识别的区域一致，表明OCT信号中的相位变化可能与相应的血流动力学变化相关。在本研究中观察并监测了触须刺激期间桶状皮质深处的局部组织动力学。
