Silverman Scott K
Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.
RNA. 2003 Apr;9(4):377-83. doi: 10.1261/rna.2200903.
Switches and sensors play important roles in our everyday lives. The chemical properties of RNA make it amenable for use as a switch or sensor, both artificially and in nature. This review focuses on recent advances in artificial RNA switches and sensors. Researchers have been applying classical biochemical principles such as allostery in elegant ways that are influencing the development of biosensors and other applications. Particular attention is given here to allosteric ribozymes (aptazymes) that are regulated by small organic molecules, by proteins, or by oligonucleotides. Also discussed are ribozymes whose activities are controlled by various nonallosteric strategies.
开关和传感器在我们的日常生活中发挥着重要作用。RNA的化学特性使其无论是在人工合成还是自然状态下，都适合用作开关或传感器。本综述聚焦于人工RNA开关和传感器的最新进展。研究人员一直在以巧妙的方式应用诸如变构等经典生化原理，这些方式正在影响生物传感器及其他应用的发展。这里特别关注受小分子、蛋白质或寡核苷酸调控的变构核酶（适体酶）。还讨论了其活性由各种非变构策略控制的核酶。
