Famulok Michael, Verma Sandeep
Institut für Biochemie, Rheinische Friedrich-Wilhelms-Universität Bonn, Gerhard-Domagk-Strasse 1, D-53121 Bonn, Germany.
Trends Biotechnol. 2002 Nov;20(11):462-6. doi: 10.1016/s0167-7799(02)02063-2.
Non-natural, functional RNA molecules, such as short interfering (si) RNAs, aptazymes, maxizymes and intramers, allow modulation of gene function at the mRNA or protein level. This review discusses recent advances made in the expression and application of these functional RNAs and illustrates how engineered, intracellularly active RNAs can serve as promising tools for understanding the function of genes and their protein products or as potential therapeutic agents.
非天然的功能性RNA分子，如小干扰（si）RNA、适体酶、大核酶和内含子酶，能够在mRNA或蛋白质水平上调节基因功能。本综述讨论了这些功能性RNA在表达和应用方面的最新进展，并阐述了经过工程改造的细胞内活性RNA如何能够作为理解基因及其蛋白质产物功能的有前景的工具，或作为潜在的治疗剂。
